Construct design for CRISPR/Cas-based genome editing in plants

被引：70

作者：

Hassan, Md Mahmudul ^{[1
,2
,3
]}

Zhang, Yingxiao ^{[4
]}

Yuan, Guoliang ^{[1
,2
]}

De, Kuntal ^{[1
]}

Chen, Jin-Gui ^{[1
,2
]}

Muchero, Wellington ^{[1
,2
]}

Tuskan, Gerald A. ^{[1
,2
]}

Qi, Yiping ^{[4
,5
]}

Yang, Xiaohan ^{[1
,2
]}

机构：

[1] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA

[2] Oak Ridge Natl Lab, Ctr Bioenergy Innovat, Oak Ridge, TN 37831 USA

[3] Patuakhali Sci & Technol Univ, Dept Genet & Plant Breeding, Dumki 8602, Patuakhali, Bangladesh

[4] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA

[5] Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD 20850 USA

来源：

TRENDS IN PLANT SCIENCE | 2021年 / 26卷 / 11期

基金：

美国国家科学基金会;

关键词：

HIGHLY EFFICIENT; GUIDE RNA; MULTIPLEX; DNA; MUTAGENESIS; RICE; ENDONUCLEASE; ARABIDOPSIS; EXPRESSION; CLEAVAGE;

D O I：

10.1016/j.tplants.2021.06.015

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

CRISPR construct design is a key step in the practice of genome editing, which includes identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and selection of regulatory elements to express gRNAs and Cas proteins. Here, we review the choices of CRISPR-based genome editors suited for different needs in plant genome editing applications. We consider the technical aspects of gRNA design and the associated computational tools. We also discuss strategies for the design of multiplex CRISPR constructs for highthroughput manipulation of complex biological processes or polygenic traits. We provide recommendations for different elements of CRISPR constructs and discuss the remaining challenges of CRISPR construct optimization in plant genome editing.

引用

页码：1133 / 1152

页数：20

共 50 条

[41] A CRISPR/Cas9 toolkit for multiplex genome editing in plants
Xing, Hui-Li
Dong, Li
Wang, Zhi-Ping
Zhang, Hai-Yan
Han, Chun-Yan
Liu, Bing
Wang, Xue-Chen
Chen, Qi-Jun
BMC PLANT BIOLOGY, 2014, 14
[42] CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks
Delight Hwarari
Yasmina Radani
Yongchao Ke
Jinhui Chen
Liming Yang
Functional & Integrative Genomics, 2024, 24
[43] CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks
Hwarari, Delight
Radani, Yasmina
Ke, Yongchao
Chen, Jinhui
Yang, Liming
FUNCTIONAL & INTEGRATIVE GENOMICS, 2024, 24 (02)
[44] Targeted genome editing in crop plants using a CRISPR/Cas system
Science Foundation in China, 2013, (02) : 16 - 16
[45] Design of Guide RNA for CRISPR/Cas Plant Genome Editing
Gerashchenkov, G. A.
Rozhnova, N. A.
Kuluev, B. R.
Kiryanova, O. Yu
Gumerova, G. R.
Knyazev, A., V
Vershinina, Z. R.
Mikhailova, E., V
Chemeris, D. A.
Matniyazov, R. T.
Baimiev, An Kh
Gubaidullin, I. M.
Baimiev, Al Kh
Chemeris, A., V
MOLECULAR BIOLOGY, 2020, 54 (01) : 24 - 42
[46] Engineering drought tolerance in plants through CRISPR/Cas genome editing
Joshi, Raj Kumar
Bharat, Suhas Sutar
Mishra, Rukmini
3 BIOTECH, 2020, 10 (09)
[47] Engineering drought tolerance in plants through CRISPR/Cas genome editing
Raj Kumar Joshi
Suhas Sutar Bharat
Rukmini Mishra
3 Biotech, 2020, 10
[48] A CRISPR/Cas9 toolkit for multiplex genome editing in plants
Hui-Li Xing
Li Dong
Zhi-Ping Wang
Hai-Yan Zhang
Chun-Yan Han
Bing Liu
Xue-Chen Wang
Qi-Jun Chen
BMC Plant Biology, 14
[49] High-efficiency CRISPR/Cas-based editing of Phalaenopsis orchid MADS genes
Tong, Chii-Gong
Wu, Fu-Hui
Yuan, Yu-Hsuan
Chen, Yan-Ru
Lin, Choun-Sea
PLANT BIOTECHNOLOGY JOURNAL, 2020, 18 (04) : 889 - 891
[50] CRISPR/Cas-Based Gene Editing Strategies for DOCK8 Immunodeficiency Syndrome
Ravendran, Sujan
Hernandez, Sabina Sanchez
Konig, Saskia
Bak, Rasmus O.
FRONTIERS IN GENOME EDITING, 2022, 4

← 1 2 3 4 5 →