Cloning, purification, crystallization and preliminary X-ray diffraction analysis of mouse PACSIN 3 protein

PACSIN-family proteins are cytoplasmic proteins that have vesicle-transport, membrane-dynamics, actin-reorganization and microtubule activities. Here, the N-terminal F-BAR domain of mouse PACSIN 3, which contains 341 amino acids, was successfully cloned, purified and crystallized. The crystal of PACSIN 3 (1341) diffracted to 2.6 angstrom resolution and belonged to space group P21, with unit-cell parameters a = 46.9, b = 54.7, c = 193.7 angstrom, a = 90, beta = 96.9, = 90 degrees. These data should provide further information on PACSIN-family protein structures.