Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)α-hydroxysteroid dehydrogenase

被引：3
|
作者：
El-Kabbani, O [1 ]
Ishikura, S
Wagner, A
Schulze-Briese, C
Hara, A
机构：
[1] Monash Univ, Victorian Coll Pharm, Dept Med Chem, Parkville, Vic 3052, Australia
[2] Gifu Pharmaceut Univ, Biochem Lab, Gifu 5028585, Japan
[3] Paul Scherrer Inst, Swiss Light Source, CH-5232 Villigen, Switzerland
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷
关键词：
D O I：
10.1107/S1744309105018427
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The 3(17) alpha-hydroxysteroid dehydrogenase from mouse is involved in the metabolism of oestrogens, androgens, neurosteroids and xenobiotic compounds. The enzyme was crystallized by the hanging-drop vapour-diffusion method in space group P222(1), with unit-cell parameters a = 84.91, b = 84.90, c = 95.83 A. The Matthews coefficient (V-M) and the solvent content were 2.21 angstrom(3) Da(-1) and 44.6%, respectively, assuming the presence of two molecules in the asymmetric unit. Diffraction data were collected to a resolution of 1.8 angstrom at the Swiss Light Source beamline X06SA using a MAR CCD area detector and gave a data set with an overall R-merge of 6.8% and a completeness of 91.1%.
引用
收藏
页码：688 / 690
页数：3
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