Exposure to human blood inactivates swine endothelial ecto-5′-nucleotidase

被引：3

作者：

Khalpey, Z ^{[1
]}

Kalsi, K

Yuen, A

Karbowska, J

Kochan, Z

Slominska, EM

Forni, M

Bacci, M

Macherini, M

Batten, P

Lavitrano, M

Yacoub, MH

Smolenski, RT

机构：

[1] Harefield Hosp, Imperial Coll, Heart Sci Ctr, London, England

[2] Med Univ Gdansk, Dept Biochem, PL-80211 Gdansk, Poland

[3] Univ Bologna, Dept Vet Morphophisiol & Anim Prod, Bologna, Italy

[4] Univ Siena, Inst Thorac & Cardiovasc Surg, I-53100 Siena, Italy

[5] Univ Milan, Dept Surg Sci & Intens Care, Milan, Italy

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2005年 / 24卷 / 04期

关键词：

ecto-5 '-nucleotidease; xenotransplantation; nucleotides; endothelial cells; complement;

D O I：

10.1081/NCN-200059707

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ecto-5'-nucleotidase (E5'N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of E5'N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5'N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5'N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of E5'N decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.

引用

页码：271 / 274

页数：4

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