Mechanisms underlying enhanced IgA production in Peyer's patch cells by membrane vesicles derived from Lactobacillus sakei

被引：19

作者：

Miyoshi, Yuki ^{[1
,2
,3
]}

Saika, Azusa ^{[2
,3
,4
]}

Nagatake, Takahiro ^{[2
,3
]}

Matsunaga, Ayu ^{[2
,3
,5
]}

Kunisawa, Jun ^{[2
,3
,4
]}

Katakura, Yoshio ^{[6
]}

Yamasaki-Yashiki, Shino ^{[2
,3
,6
]}

机构：

[1] Kansai Univ, Grad Sch Sci & Engn, Chem Mat & Bioengn Major, Suita, Osaka, Japan

[2] Natl Inst Biomed Innovat Hlth & Nutr, Lab Vaccine Mat, Ctr Vaccine & Adjuvant Res, Ibaraki, Osaka, Japan

[3] Natl Inst Biomed Innovat Hlth & Nutr, Lab Gut Environm Syst, Ibaraki, Osaka, Japan

[4] Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka, Japan

[5] Azabu Univ, Sch Life & Environm Sci, Dept Food & Life Sci, Sagamihara, Kanagawa, Japan

[6] Kansai Univ, Fac Chem Mat & Bioengn, Dept Life Sci & Biotechnol, Suita, Osaka, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2021年 / 85卷 / 06期

基金：

日本学术振兴会;

关键词：

lactic acid bacteria; membrane vesicle; gut immunity; IgA; Peyer's patches; LACTIC-ACID BACTERIA; DENDRITIC CELLS; SECRETION;

D O I：

10.1093/bbb/zbab065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analyzed the mechanisms underlying enhanced IgA production in the cells of Peyer's patch cells via membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC 15893. Depletion of CD11c(+) cells from Peyer's patch cells suppressed the enhanced IgA production mediated by membrane vesicles. Meanwhile, the stimulation of bone-marrow-derived dendritic cells with membrane vesicles increased gene expression of inducible nitric oxide synthase, retinaldehyde dehydrogenase 2, and several inflammatory cytokines. The production of nitric oxide and interleukin (IL)-6 by membrane vesicle stimulation was induced via Toll-like receptor 2 on bone marrow-derived dendritic cells. Inhibition of inducible nitric oxide synthase and retinaldehyde dehydrogenase 2, as well as the neutralization of IL-6 in Peyer's patch cells, suppressed the enhanced IgA production by membrane vesicle stimulation. Hence, nitric oxide, retinoic acid, and IL-6 induced by membrane vesicles play crucial roles in the enhanced IgA production elicited by membrane vesicles in Peyer's patch cells.

引用

页码：1536 / 1545

页数：10

共 25 条

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[22] ACTIVATION OF PHOSPHOLIPASE-C (PLC) IN PERMEABILIZED MEMBRANE-VESICLES DERIVED FROM RBL CELLS - ACTIVATION BY FC-EPSILON-RI AND NA3VO4 BUT NOT BY GTP-GAMMA-S IS ENHANCED BY THE ADDITION OF PHOSPHOENOLPYRUVATE
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