Analysis of hybridoma cell culture processes by SDS/gel capillary electrophoresis and matrix-assisted laser desorption ionization-time-of-flight MS

被引：0

作者：

Klyushnichenko, V

Rodenbrock, A

Thommes, J

Kula, MR ^{[1
]}

Heine, H

Biselli, M

机构：

[1] Heinrich Heine Univ, Inst Enzymtechnol, D-52426 Julich, Germany

[2] Forschungszentrum Julich, Inst Biotechnol, D-52425 Julich, Germany

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 1998年 / 27卷

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SDS/gel capillary electrophoresis (SDS/gel CE) and matrix-assisted laser desorption ionization-time-of-flight MS (MALDI-TOF-MS) were employed to analyse changes in the culture broth during batch and continuous cultivation of hybridoma cells, The stability of IgG was analysed by SDS/gel CE and capillary zone electrophoresis (CZE), The results obtained by the new analytical procedures reflect the changes in the cultivation conditions very well, indicating that these tools can be used to follow animal cell culture processes. A new technique to collect fractions of very small volumes during the CZE separation is described, and the successful off-line coupling of CZE and MALDI-TOF-MS for the analysis of biotechnological processes is demonstrated.

引用

页码：181 / 188

页数：8

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