The molecular genetic differentiation of cultured Saccharomyces strains

被引：16
|
作者：
Naumova, ES
Zholudeva, MV
Martynenko, NN
Naumov, GI
机构：
[1] State Res Inst Gnet & Select Ind Microorganisms, Moscow 117545, Russia
[2] State Univ Foodstuffs, Moscow, Russia
来源：
MICROBIOLOGY | 2005年 / 74卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
Saccharomyces; S. cerevisiae x S. bayanus var. uvarum hybrids; RFLP analysis; rDNA; molecular karyotyping;
D O I：
10.1007/s11021-005-0049-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A comparative molecular genetic study of cultured Saccharomyces strains isolated from the surface of berries and various fermentation processes showed that baker's yeast and black-currant isolates contain not only Saccharomyces cerevisiae but also S. cerevisiae x S. bayanus var. uvarum hybrids. The molecular karyotyping of baker's, brewer's, and wine yeasts showed their polyploidy. The restriction enzyme analysis of non-coding rDNA regions (5.8S-ITS and IGS2) makes it possible to differentiate species of the genus Saccharomyces and to identify interspecies hybrids. The microsatellite primer (GTG)(5) can be used to study the populations of cultured S. cerevisiae strains.
引用
收藏
页码：179 / 187
页数：9
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