Quantitative PCR determination of human cytomegalovirus in blood cells

被引:5
|
作者
Satou, J
Funato, S
Satoh, N
Abe, Y
Ishii, KK
Sasaki, T
Kaku, M
机构
[1] Tohoku Univ, Sch Med, Dept Clin Med, Div Mol Diagnost,Aoba Ku, Sendai, Miyagi 9808574, Japan
[2] Tohoku Univ, Sch Med, Dept Clin Med, Div Rheumatol & Haematol, Sendai, Miyagi 9808574, Japan
关键词
polymerase chain reaction (PCR); quantitative PCR; cytomegalovirus (CMV); liver transplantation;
D O I
10.1002/jcla.1014
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
We evaluated a rapid and sensitive method to determine human cytomegalovirus (CMV) DNA levels in blood cells using a quantitative polymerase chain reaction (PCR) technique. This method is based on real-time detection of PCR using a dual fluorescence-labeled probe and a sequence detector. Ten copies of CMV DNA were detected, when 1 mug of DNA from blood samples was used with this method, and a good correlation was obtained between increased concentrations of copy numbers calculated and measured copy numbers of CMV DNA (r = 0.999). Forty normal subjects exhibited no copies of CMV DNA. On the other hand, a 6-month-old girl tested positive for increased levels 4 weeks after liver transplant. This method is simple, accurate, and sensitive for the quantitative detection of CMV DNA in vivo, indicating possible applications for the diagnosis and monitoring of CMV infection. J. Clin. Lab. Anal. 15:122-126, 2001. (C) 2001 Wiley-Liss, Inc.
引用
收藏
页码:122 / 126
页数:5
相关论文
共 50 条
  • [31] Lack of Human Cytomegalovirus in Gliomas. A Viral Load Analysis by Quantitative Real Time-PCR
    Garcia-Martinez, A.
    Irles, E.
    Barbera, V. M.
    Egoavil, C.
    Alenda, C.
    Castillejo, A.
    Castillejo, M. I.
    Ochoa, E.
    Barea, M. C.
    Quintanar, T.
    Heredia, L.
    Moreno, P.
    Herrero, J.
    Portugues, M. J.
    Culianez, A.
    Andrada, E.
    Sendra, J.
    Rodriguez-Lescure, A.
    Paya, A.
    Aranda, I.
    Soto, J. L.
    MODERN PATHOLOGY, 2013, 26 : 416A - 416A
  • [32] Lack of Human Cytomegalovirus in Gliomas. A Viral Load Analysis by Quantitative Real Time-PCR
    Garcia-Martinez, A.
    Irles, E.
    Barbera, V. M.
    Egoavil, C.
    Alenda, C.
    Castillejo, A.
    Castillejo, M. I.
    Ochoa, E.
    Barea, M. C.
    Quintanar, T.
    Heredia, L.
    Moreno, P.
    Herrero, J.
    Portugues, M. J.
    Culianez, A.
    Andrada, E.
    Sendra, J.
    Rodriguez-Lescure, A.
    Paya, A.
    Aranda, I.
    Soto, J. L.
    LABORATORY INVESTIGATION, 2013, 93 : 416A - 416A
  • [33] Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR
    Yamaguchi, Makoto
    Kawada, Jun-ichi
    Torii, Yuka
    Haruta, Kazunori
    Suzuki, Takako
    Horiba, Kazuhiro
    Takahashi, Yoshiyuki
    Ito, Yoshinori
    JOURNAL OF MEDICAL VIROLOGY, 2022, 94 (09) : 4559 - 4564
  • [34] Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR
    Roberts, TC
    Buller, RS
    GaudreaultKeener, M
    Sternhell, KE
    Garlock, K
    Singer, GG
    Brennan, DC
    Storch, GA
    JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (09) : 2224 - 2228
  • [35] Whole blood real-time quantitative PCR for cytomegalovirus infection follow-up in transplant recipients
    Garrigue, I
    Boucher, S
    Couzi, L
    Caumont, A
    Dromer, C
    Neau-Cransac, M
    Tabrizi, R
    Schrive, MH
    Fleury, H
    Lafon, ME
    JOURNAL OF CLINICAL VIROLOGY, 2006, 36 (01) : 72 - 75
  • [36] IMMUNOCYTOLOGICAL DETERMINATION OF LYSOZYME IN HUMAN BLOOD CELLS
    ASAMER, H
    SCHMALZL, F
    BRAUNSTEINER, H
    ACTA HAEMATOLOGICA, 1969, 41 (01) : 49 - +
  • [37] Quantitative Determination of Topiramate in Human Serum and Umbilical Cord Blood
    Cifuentes, Cristina
    Mennickent, Sigrid
    De Diego, Marta
    CURRENT PHARMACEUTICAL ANALYSIS, 2019, 15 (05) : 521 - 527
  • [38] QUANTITATIVE-DETERMINATION OF CATECHOLAMINES IN HUMAN RED BLOOD-CELLS BY GAS-LIQUID-CHROMATOGRAPHY
    LOVELADY, HG
    BIOCHEMICAL MEDICINE, 1976, 15 (02): : 138 - 144
  • [39] Quantitative PCR for Detection of Babesia microti in Ixodes scapularis Ticks and in Human Blood
    Rollend, Lindsay
    Bent, Stephen J.
    Krause, Peter J.
    Usmani-Brown, Sahar
    Steeves, Tanner K.
    States, Sarah L.
    Lepore, Timothy
    Ryan, Raymond
    Dias, Fil
    Ben Mamoun, Choukri
    Fish, Durland
    Diuk-Wasser, Maria A.
    VECTOR-BORNE AND ZOONOTIC DISEASES, 2013, 13 (11) : 784 - 790
  • [40] Telomere length analysis of human mesenchymal stem cells by quantitative PCR
    Samsonraj, Rebekah M.
    Raghunath, Michael
    Hui, James H.
    Ling, Ling
    Nurcombe, Victor
    Cool, Simon M.
    GENE, 2013, 519 (02) : 348 - 355