Trypanosoma cruzi epimastigotes express the ouabain- and vanadate-sensitive (Na++K+)ATPase activity

被引：0

作者：

Caruso-Neves, C

Einicker-Lamas, M

Chagas, C

Oliveira, MM

Vieyra, A

Lopes, AG ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol Renal, BR-21941 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Biomembranas, BR-21941 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Dept Bioquim Med, Rio De Janeiro, Brazil

来源：

ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES | 1998年 / 53卷 / 11-12期

关键词：

(Na++K+)ATPase; Trypanosoma cruzi; ATPase; epimastigote; ouabain;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of (Na++K+)ATPase activity in CL14 clone and NIH NTY strain of Trypanosoma cruzi epimastigotes is demonstrated. A Na+ plus K+ stimulated ATPase activity is found in both strains. The optimal Na+/K+ ratio is 5:1 and 9:1 in CL14 clone and NIH NTY strain, respectively. In both strains, vanadate completely inhibits the ouabain-sensitive ATPase activity indicating that it belongs to the P-type (E-1/E-2) family of ion-transporting ATPases. The I-50 for vanadate is 0.66 +/- 0.04 and 0.01 +/- 0.02 mu M in CL14 clone and NIH NTY strain, respectively. These data indicate that both strains of T. cruzi epimastigotes express the ouabain- and vanadate-sensitive (Na++K+)ATPase activity. On the other hand, the discrepancy between the parameters analyzed for the inhibitors suggests that they express different isoforms of this enzyme.

引用

页码：1049 / 1054

页数：6

共 50 条

[41] [H-3] OUABAIN BINDING-SITES AND (NA++K+)ATPASE ACTIVITY IN HEART OF RATS FED CHOLESTEROL
REN, YF
ALAM, BS
ALAM, SQ
FEDERATION PROCEEDINGS, 1986, 45 (04) : 843 - 843
[42] 2 DIFFERENT INHIBITORY SITES ON (NA++K+)-ATPASE DIFFERENTIATED BY CHLORPROMAZINE FREE RADICAL AND OUABAIN
BRODY, TM
AKERA, T
PHARMACOLOGIST, 1969, 11 (02): : 269 - &
[43] Molecular cloning and characterization of ouabain-insensitive Na+-ATPase in the parasitic protist, Trypanosoma cruzi
Iizumi, Kyoichi
Mikami, Yuko
Hashimoto, Muneaki
Nara, Takeshi
Hara, Yukichi
Aoki, Takashi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2006, 1758 (06): : 738 - 746
[44] STIMULATION OF [H-3] OUABAIN BINDING TO RAT SYNAPTOSOMAL (NA++K+)-ATPASE BY ALUMINUM
CASPERS, ML
DOW, MJ
KWAISER, TM
FASEB JOURNAL, 1991, 5 (04): : A752 - A752
[45] OUABAIN-INDUCED INHIBITION OF CARDIAC (NA++K+)-ATPASE AND POSITIVE INOTROPIC RESPONSE - DISCUSSION
SCHWARTZ, A
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1974, 242 (NOV29) : 683 - 683
[46] INFLUENCE OF ASSAY PROCEDURES ON STUDY OF OUABAIN-INHIBITION OF (NA++K+)-ATPASE IN-VITRO
AKERA, T
TOBIN, T
BRODY, TM
PHARMACOLOGIST, 1971, 13 (02): : 308 - &
[47] OUABAIN-BINDING AND PHOSPHORYLATION OF (NA++K+)ATPASE TREATED WITH N-ETHYLMALEIMIDE OR OLIGOMYCIN
HEGYVARY, C
BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 422 (02) : 365 - 379
[48] PURIFICATION AND CHARACTERIZATION OF (NA++K+)-ATPASE .1. INFLUENCE OF DETERGENTS ON ACTIVITY OF (NA++K+)-ATPASE IN PREPARATIONS FROM OUTER MEDULLA OF RABBIT KIDNEY
JORGENSEN, PL
SKOU, JC
BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 233 (02) : 366 - +
[49] DO FACTORS IN ISCHEMIC BRAIN SUPPRESS (NA++K+)-ATPASE ACTIVITY
MERCER, LF
BERTONI, JM
STROKE, 1982, 13 (01) : 118 - 118
[50] THE EFFECTS OF ACTH4-10 ON NA++K+ ATPASE ACTIVITY
MORGAN, K
SPURLOCK, G
SOUTHGATE, K
MIR, MA
CLINICAL SCIENCE, 1984, 67 : P79 - P79

← 1 2 3 4 5 →