Evaluation of performances of VERSANT HCV RNA 1.0 assay (kPCR) and Roche COBAS AmpliPrep/COBAS TaqMan HCV test v2.0 at low level viremia

被引：0
|
作者：
Mazzuti, Laura [1 ]
Lozzi, Maria Antonietta [2 ]
Riva, Elisabetta [3 ]
Maida, Paola [1 ]
Falasca, Francesca [1 ]
Antonelli, Guido [1 ]
Turriziani, Ombretta [1 ]
机构：
[1] Sapienza Univ Rome, Dept Mol Med, Rome, Italy
[2] Sapienza Univ Rome, Dept Publ Hlth & Infect Dis, Rome, Italy
[3] Univ Campus Biomed Rome, Rome, Italy
来源：
NEW MICROBIOLOGICA | 2016年 / 39卷 / 03期
关键词：
HCV; Viral load; Real-time PCR; VERSANT HCV RNA; COBAS TaqMan HCV; RESIDUAL VIREMIA; THERAPY;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We assess the concordance between low level HCV values obtained using the VERSANT HCV RNA 1.0 Assay (kPCR) and COBAS AmpliPrep/COBAS TaqMan HCV Quantitative Test v2.0. The correlation between the values obtained by the two RT-PCR assays for samples with quantifiable HCV RNA levels revealed that viral load measured by kPCR significantly correlated with that of the CAP/CTM (R=0.644, P<0.0001). The results show a good concordance (n=126/144, 87%); discordant results were mainly observed in the assessment of values below the lower limit of detection of the assays. These variations may have an impact on clinical decisions for patients on HCV triple therapy or interferon-free regimens. It is therefore recommended to monitor individual patients with the same test throughout treatment.
引用
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页码：224 / 227
页数：4
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