Interaction of two flavonols with fat mass and obesity-associated protein investigated by fluorescence quenching and molecular docking

被引：21
|
作者：
Wang, Zechun [1 ]
Wang, Ning [1 ]
Han, Xinxin [1 ]
Wang, Ruiyong [1 ]
Chang, Junbiao [1 ]
机构：
[1] Zhengzhou Univ, Coll Chem & Mol Engn, 100 Sci Ave, Zhengzhou 450001, Henan, Peoples R China
来源：
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 2018年 / 36卷 / 13期
基金：
中国国家自然科学基金;
关键词：
binding; flavonols; FTO; fluorescence; molecular docking; HUMAN SERUM-ALBUMIN; GEMINI SURFACTANT; FTO PROTEIN; IN-VITRO; BOVINE; BINDING; SPECTROSCOPY; DERIVATIVES; GENE; ANTIBACTERIAL;
D O I：
10.1080/07391102.2017.1388287
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The binding of two flavonols with fat mass and obesity-associated protein (FTO) was studied using fluorescence spectroscopy, Stern-Volmer kinetics, UV-Vis absorption, and molecular docking. The quenching of FTO fluorescence was determined to be static with binding constants on the order of 10(4) M-1. The interaction was studied over three temperatures, and the binding was found to be exothermic with a positive change in entropy. Thermodynamic analysis and molecular modeling suggest that hydrophobic interaction and hydrogen bonding interaction are the main binding force in stabilizing the flavonol-FTO complex.
引用
收藏
页码：3388 / 3397
页数：10
相关论文
共 50 条
  • [21] Genetic Associations of Obesity: The Fat-Mass and Obesity-Associated (FTO) Gene
    Ehrlich, Adam C.
    Friedenberg, Frank K.
    [J]. CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY, 2016, 7
  • [22] Computational study on the interaction of flavonoids with fat mass and obesity associated protein
    Alharbi, Khalid Khalaf
    Syed, Rabbani
    Khan, Imran Ali
    [J]. JOURNAL OF ENVIRONMENTAL BIOLOGY, 2015, 36 (02): : 419 - 424
  • [23] The role of chlorine atom on the binding between acrylonitrile derivatives and fat mass and obesity-associated protein
    Bai, Ning
    Gan, Ya
    Li, Xitong
    Gao, Shuting
    Yu, Wenquan
    Wang, Ruiyong
    Chang, Junbiao
    [J]. JOURNAL OF MOLECULAR RECOGNITION, 2021, 34 (04)
  • [24] Lose Weight with Traditional Chinese Medicine? Potential Suppression of Fat Mass and Obesity-Associated Protein
    Chang, Pei-Chun
    Wang, Jing-Doo
    Lee, Min-Min
    Chang, Su-Sen
    Tsai, Tsung-Ying
    Chang, Kai-Wei
    Tsai, Fuu-Jen
    Chen, Calvin Yu-Chian
    [J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2011, 29 (03): : 471 - 483
  • [25] Ubiquitination Regulates the Proteasomal Degradation and Nuclear Translocation of the Fat Mass and Obesity-Associated (FTO) Protein
    Zhu, Tianyi
    Yong, Xuan Ling Hilary
    Xia, Di
    Widagdo, Jocelyn
    Anggono, Victor
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2018, 430 (03) : 363 - 371
  • [26] Identification of nafamostat mesilate as an inhibitor of the fat mass and obesity-associated protein (FTO) demethylase activity
    Han, Xinxin
    Wang, Ning
    Li, Junya
    Wang, Ying
    Wang, Ruiyong
    Chang, Junbiao
    [J]. CHEMICO-BIOLOGICAL INTERACTIONS, 2019, 297 : 80 - 84
  • [27] The protective effect of the obesity-associated rs9939609 A variant in fat mass- and obesity-associated gene on depression
    Samaan, Z.
    Anand, S.
    Zhang, X.
    Desai, D.
    Rivera, M.
    Pare, G.
    Thabane, L.
    Xie, C.
    Gerstein, H.
    Engert, J. C.
    Craig, I.
    Cohen-Woods, S.
    Mohan, V.
    Diaz, R.
    Wang, X.
    Liu, L.
    Corre, T.
    Preisig, M.
    Kutalik, Z.
    Bergmann, S.
    Vollenweider, P.
    Waeber, G.
    Yusuf, S.
    Meyre, D.
    [J]. MOLECULAR PSYCHIATRY, 2013, 18 (12) : 1281 - 1286
  • [28] Identification of Clausine E as an inhibitor of fat mass and obesity-associated protein (FTO) demethylase activity
    Wang, Ying
    Li, Junya
    Han, Xinxin
    Wang, Ning
    Song, Chuanjun
    Wang, Ruiyong
    Chang, Junbiao
    [J]. JOURNAL OF MOLECULAR RECOGNITION, 2019, 32 (10)
  • [29] The protective effect of the obesity-associated rs9939609 A variant in fat mass- and obesity-associated gene on depression
    Z Samaan
    S Anand
    X Zhang
    D Desai
    M Rivera
    G Pare
    L Thabane
    C Xie
    H Gerstein
    J C Engert
    I Craig
    S Cohen-Woods
    V Mohan
    R Diaz
    X Wang
    L Liu
    T Corre
    M Preisig
    Z Kutalik
    S Bergmann
    P Vollenweider
    G Waeber
    S Yusuf
    D Meyre
    [J]. Molecular Psychiatry, 2013, 18 : 1281 - 1286
  • [30] Fat mass and obesity-associated protein regulates RNA methylation associated with depression-like behavior in mice
    Liu, Shu
    Xiu, Jianbo
    Zhu, Caiyun
    Meng, Kexin
    Li, Chen
    Han, Rongrong
    Du, Tingfu
    Li, Lanlan
    Xu, Lingdan
    Liu, Renjie
    Zhu, Wanwan
    Shen, Yan
    Xu, Qi
    [J]. NATURE COMMUNICATIONS, 2021, 12 (01)
12345