Intratumoral Switch of Molecular Phenotype and Overall Survival in Muscle Invasive Bladder Cancer

被引:1
|
作者
De Carlo, Camilla [1 ,2 ]
Valeri, Marina [1 ,2 ]
Rudini, Noemi [1 ]
Zucali, Paolo Andrea [2 ,3 ]
Cieri, Miriam [1 ]
Elefante, Grazia Maria [1 ]
D'antonio, Federica [2 ,3 ]
Hurle, Rodolfo [4 ]
Giordano, Laura [5 ]
Bressan, Alessandra [1 ,2 ]
Lazzeri, Massimo [4 ]
Perrino, Matteo [3 ]
Guazzoni, Giorgio [2 ,4 ]
Terracciano, Luigi Maria [1 ,2 ]
Colombo, Piergiuseppe [1 ,2 ]
机构
[1] IRCCS Humanitas Res Hosp, Dept Pathol, Via Manzoni 56, I-20089 Milan, Italy
[2] Humanitas Univ, Dept Biomed Sci, Via Rita Levi Montalcini 4, I-20090 Milan, Italy
[3] IRCCS Humanitas Res Hosp, Dept Oncol, Via Manzoni 56, I-20089 Milan, Italy
[4] IRCCS Humanitas Res Hosp, Dept Urol, Via Manzoni 56, I-20089 Milan, Italy
[5] IRCCS Humanitas Res Hosp, Biostat Unit, Via Manzoni 56, I-20089 Milan, Italy
关键词
bladder cancer; molecular phenotype; luminal; basal; neu-like; phenotypical transition; CD44; CK5/6; CK20; pPAR gamma; OS; PFS; UROTHELIAL CARCINOMA; URINARY-BLADDER; SUBTYPES; EXPRESSION; GRADE; BASAL;
D O I
10.3390/cancers14133256
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Simple Summary The Cancer Genome Atlas (TCGA) and more recent genome profiling recently revealed major intrinsic molecular subtypes in urothelial carcinoma (UC). Here we propose a fast and standardized immunophenotypical classification score (Piescore) that may discriminate between luminal, basal, or neu-like UC as a surrogate of molecular profile, and we describe, for the first time, an intratumoral phenotypical switch in tissue protein expression, from non-muscle to muscle-invasive progression. Our data show that a change from a luminal to a neu-like phenotype could worsen overall survival compared with a transition to a basal phenotype. In recent years, immunohistochemical protein expression was studied as a surrogate to the molecular classification of bladder cancer, although no tissue biomarkers are available for clinical use to predict survival or the response to neoadjuvant chemotherapy (CT) in UC, as the literature produced conflicting results. This retrospective study included TURB specimens harboring foci of HG pT2 muscle-invasive bladder carcinoma (MIBC) from 251 patients who subsequently underwent radical cystectomy. We performed immunohistochemical analysis on tumor samples, for relevant gene-expression-based markers for basal type (CD44, CK5/6) and luminal type (CK20 and pPAR gamma). Piescore, investigated in both non-muscle-invasive (NMI) and muscle-invasive (MI) components of the tumor, divided basal and luminal UC-types when at least three of the four markers were consistent with a specific phenotype, mixed types if one/two luminal and basal markers were present simultaneously, and neu-like types when all four markers investigated were negative. Eighteen selected cases were also investigated with RT-PCR to validate, and to increase the specificity of, the immunohistochemical results. We observe an immunophenotypical difference in the NMI and MI components in 96/251 UC patients (38.25%): half of tumors (44/96 cases) have a transition to basal, 36.46% (35/96 cases) to neu-like, 12.5% (12/96 cases) to mixed, and 5.2% (5/96 cases) to luminal phenotypes. Mixed tumors in the NMI component are more likely to change phenotype than other groups, particularly compared with basal tumors, which demonstrate greater stability (only 8/96 cases, p < 0.00001). The transition of luminal tumors to basal display a better OS compared with the transition toward neu-like tumors (p = 0.027). Overall, the phenotypical switch does not affect lymphovascular invasion, pT, DFS, or OS compared with non-switched cases. In the MI component, the presence of CD44 expression, irrespective of score-related phenotype, shows a protective effect in papillary-type UC (OS p = 0.008, HR 0.453, PFS p = 0.07, HR 0.599), and in UC naive for CT (p = 0.0479). Piescore immunophenotyping reveals an intratumoral phenotypical transition between the NMI and MI components of the same tumor. The molecular change is a common event in the mixed and luminal categories, but not in basal tumors, which show better phenotypical stability. This phenomenon could partially explain the sensitivity of a subset of luminal UC to chemotherapy: good responders could be "non-real" luminal UC, which acquire nasal markers, such as CD44.
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页数:17
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