Effect of root canal procedures on endotoxins and endodontic pathogens

被引:73
|
作者
Vianna, M. E.
Horz, H. -P.
Conrads, G.
Zaia, A. A.
Souza-Filho, F. J.
Gomes, B. P. F. A.
机构
[1] State Univ Campinas UNICAMP, Dent Sch Piracicaba, Dept Restorat Dent, BR-13414018 Piracicaba, SP, Brazil
[2] RWTH Univ Hosp, Dept Med Microbiol, Aachen, Germany
[3] RWTH Univ Hosp, Dept Operat & Prevent Dent & Periodontol, Div Oral Microbiol & Immunol, Aachen, Germany
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 2007年 / 22卷 / 06期
关键词
calcium hydroxide; chlorhexidine; endotoxin; microbial reduction;
D O I
10.1111/j.1399-302X.2007.00379.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background/aims: The purpose of this study was to determine the amount of endotoxin (lipopolysaccharide) and cultivable bacteria in human necrotic root canals before (S 1) and after chemo-mechanical preparation using chlorhexidine (CHX) gel as auxiliary chemical substance (S2), and after 7 days of intracanal dressing (S3) in order to evaluate the antiendotoxin and antimicrobial effects of endodontic procedures. Method: Twenty-four teeth were selected for the present study. Chemo-mechanical preparation was performed using 2% CHX gel and three different intracanal medicaments [Ca(OH)(2) paste; 2% CHX gel; and Ca(OH)(2) + 2% CHX gel]. A quantitative chromogenic Limulus amoebocyte lysate assay was used to measure the amount of endotoxin. Aerobic and anaerobic techniques were used to isolate and identify bacteria, and to determine the bacterial reduction by counting colony-forming units (CFU). Results: Endotoxins and bacteria were present in 100% of the initial samples, with endotoxin concentration ranging from 62.93 to 214.56 UE/ml and CFU ranging from 4 x 10(5) to 2.6 x 10(6). After chemo-mechanical preparation a mean endotoxin reduction of 44.4% was found. Fight (33.3%) root canals were still positive by culture analysis with a mean reduction of bacteria (CFU) of 99.96%. After 7 days of intracanal dressing, endotoxin concentration decreased by only 1.4% compared with S2, and residual bacteria were recovered by culture analysis in 13 cases (54.1%). No significant difference was found among different intracanal medicaments. Conclusion: Relatively high values of endotoxin were still present in the root canal after chemo-mechanical preparation although the majority of bacteria were eliminated. No improvement was achieved by 7 days of intracanal dressing.
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页码:411 / 418
页数:8
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