Concurrent isolation of hepatic stem cells and hepatocytes from the human liver

被引：3
|
作者：
Lee, Serene M. L. [1 ]
Bertinetti-Lapatki, Cristina [2 ]
Schiergens, Tobias S. [1 ]
Jauch, Karl-Walter [3 ]
Roth, Adrian B. [2 ]
Thasler, Wolfgang E. [1 ,4 ]
机构：
[1] Ludwig Maximilians Univ Munchen, Dept Gen Visceral & Transplant Surg, 5H 02 Room 428,Marchioninistr 15, D-81377 Munich, Germany
[2] F Hoffmann La Roche Ltd, Roche Innovat Ctr, Pharmaceut Sci, Grenzacherstr 124, CH-4070 Basel, Switzerland
[3] Ludwig Maximilians Univ Munchen, Med Directorate, Marchioninistr 15, D-81377 Munich, Germany
[4] Red Cross Hosp Munich, Dept Gen Visceral & Minimally Invas Surg, Nymphenburger Str 163, D-80634 Munich, Germany
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2020年 / 56卷 / 03期
关键词：
Hepatic stem cells; Hepatic progenitor cells; Hepatocytes; Liver cell isolation; Liver in vitro model;
D O I：
10.1007/s11626-020-00433-w
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Hepatocytes differentiated from induced pluripotent stem cells or stem cells have the potential to be representative in vitro models of the human liver for research as well as early safety assessment programs. However, up until now, there has been no definitive proof that differentiated hepatocytes recapitulate the phenotype and functional characteristics of primary hepatocytes from the same individual. Thus, a method for the concurrent isolation of hepatocytes and hepatic stem cells is presented here to provide the cells necessary for the evaluation of the required benchmarking. The method presented here generated high-quality hepatocytes with a purity of 94 +/- 1% and a high percentage viability of 79 +/- 2%. Furthermore, the hepatic stem cells isolated were found to be actively proliferating and have a purity of 98 +/- 1%. Thus, these isolated cells can be used as a powerful tool for the validation of differentiated hepatocyte in vitro models.
引用
收藏
页码：253 / 260
页数：8
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