Age related changes in cell stiffness of tendon stem/progenitor cells and a rejuvenating effect of ROCK-inhibition

被引：23
|
作者：
Kiderlen, Stefanie [1 ,2 ,3 ]
Polzer, Christoph [2 ,4 ]
Raedler, Joachim O. [2 ,3 ]
Docheva, Denitsa [5 ]
Clausen-Schaumann, Hauke [1 ,3 ]
Sudhop, Stefanie [1 ,3 ]
机构：
[1] Munich Univ Appl Sci, Ctr Appl Tissue Engn & Regenerat Med CANTER, Munich, Germany
[2] Ludwig Maximilians Univ Munchen, Fac Phys, Soft Condensed Matter, Munich, Germany
[3] Ludwig Maximilians Univ Munchen, Ctr NanoSci, Munich, Germany
[4] Munich Univ Appl Sci, Multiphoton Imaging Lab, Munich, Germany
[5] Univ Regensburg, Dept Trauma Surg, Expt Trauma Surg, Med Ctr, Regensburg, Germany
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2019年 / 509卷 / 03期
关键词：
hTSPC; Elastic modulus; Cell aging; ROCK-inhibition; Rejuvenation; MESENCHYMAL STEM-CELLS; MECHANICAL-PROPERTIES; SELF-RENEWAL; IN-VITRO; FIBROBLASTS; ORGANIZATION; ELASTICITY; SENESCENCE; GROWTH;
D O I：
10.1016/j.bbrc.2019.01.027
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tendon stem/progenitor cells (TSPC) are potential targets for regenerative medicine and the treatment of tendon injuries. The frequency of such injuries increases in elderly patients while the proportion of functional TSPCs in tendon tissue decreases, protracting tendon repair. Using atomic force microscopy (AFM), we show that cell stiffness and size increase in TSPCs isolated from elderly patients (A-TSPC) compared to TSPCs from younger patients (Y-TSPC). Additionally, two-photon excited fluorescence (TPEF) microscopy revealed a denser, well-structured actin cytoskeleton in A-TSPC, which correlates with the augmented cell stiffness. Treating A-TSPC with ROCK-inhibitor, reverses these age-related changes, and has rejuvenating effect on cell morphology and stiffness. We assume that cellular stiffness is a suitable marker for cell aging and ROCK a potential target for therapeutic applications of cell rejuvenation. (C) 2019 The Authors. Published by Elsevier Inc.
引用
收藏
页码：839 / 844
页数：6
相关论文
共 50 条
  • [41] PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells
    Tsung-Chuan Ho
    Shawn H. Tsai
    Shu-I Yeh
    Show-Li Chen
    Kwang-Yi Tung
    Hsin-Yu Chien
    Yung-Chang Lu
    Chang-Hung Huang
    Yeou-Ping Tsao
    Stem Cell Research & Therapy, 10
  • [42] Circulating Endothelial Progenitor Cells and Age-Related White Matter Changes
    Jickling, Glen
    Salam, Abdul
    Mohammad, Askar
    Hussain, Muhammad S.
    Scozzafava, James
    Nasser, Abdul Majeed
    Jeerakathil, Thomas
    Shuaib, Ashfaq
    Camicioli, Richard
    STROKE, 2009, 40 (10) : 3191 - 3196
  • [43] Age-related Changes in Cell Adhesion Molecule, Progenitor Cells, And Vascularity in Human Hippocampus in Major Depression
    Boldrini, Maura
    Santiago, Adrienne
    Butt, Tanya
    Dwork, Andrew
    Rosoklija, Gorazd
    Arango, Victoria
    Hen, Rene'
    Mann, J. John
    NEUROPSYCHOPHARMACOLOGY, 2014, 39 : S325 - S325
  • [44] Perivascular cells of the supraspinatus tendon express both tendon- and stem cell-related markers
    Tempfer, Herbert
    Wagner, A.
    Gehwolf, R.
    Lehner, C.
    Tauber, M.
    Resch, H.
    Bauer, H. C.
    HISTOCHEMISTRY AND CELL BIOLOGY, 2009, 131 (06) : 733 - 741
  • [45] Perivascular cells of the supraspinatus tendon express both tendon- and stem cell-related markers
    Herbert Tempfer
    A. Wagner
    R. Gehwolf
    C. Lehner
    M. Tauber
    H. Resch
    H. C Bauer
    Histochemistry and Cell Biology, 2009, 131 : 733 - 741
  • [46] Bone marrow mesenchymal stem cell-derived exosomes promote tendon regeneration by facilitating the proliferation and migration of endogenous tendon stem/progenitor cells
    Yu, Huilei
    Cheng, Jin
    Shi, Weili
    Ren, Bo
    Zhao, Fengyuan
    Shi, Yuanyuan
    Yang, Peng
    Duan, Xiaoning
    Zhang, Jiying
    Fu, Xin
    Hu, Xiaoqing
    Ao, Yingfang
    ACTA BIOMATERIALIA, 2020, 106 : 328 - 341
  • [47] Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review
    El-Rashidy, Aiah A.
    El Moshy, Sara
    Radwan, Israa Ahmed
    Rady, Dina
    Abbass, Marwa M. S.
    Doerfer, Christof E.
    Fawzy El-Sayed, Karim M.
    POLYMERS, 2021, 13 (17)
  • [48] Age-Related Changes in the Inflammatory Status of Human Mesenchymal Stem Cells: Implications for Cell Therapy
    Zhang, Ying
    Ravikumar, Maanasa
    Ling, Ling
    Nurcombe, Victor
    Cool, Simon M.
    STEM CELL REPORTS, 2021, 16 (04): : 694 - 707
  • [49] Age-related changes in the energy of human mesenchymal stem cells
    Barilani, Mario
    Lovejoy, Christopher
    Piras, Roberta
    Abramov, Andrey Y.
    Lazzari, Lorenza
    Angelova, Plamena R.
    JOURNAL OF CELLULAR PHYSIOLOGY, 2022, 237 (03) : 1753 - 1767
  • [50] Age-related changes in adipose tissue derived stem cells
    Hasegawa, S.
    Akamatsu, H.
    Yamamoto, N.
    Yamada, T.
    Yoshimura, T.
    Hasebe, Y.
    Inoue, Y.
    Mizutani, H.
    Masunaga, K.
    Nakata, S.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2008, 128 : S145 - S145
12345