Construction of the starter to produce lignocellulases via solid state fermentation of metropolis lignocellulosic waste defoliation

The study demonstrates the starter construction and its solid state fermentation (SSF) using metropolis lignocellulosic waste defoliation supplemented with wheat bran as substrates for lignocellulases production. The starter is constructed by natural microflora growing on defoliation additionally reinforced with Aspergillus niger ZJU-RYD1, using the SSF substrate defoliation with 90% moisture content, successfully produced the integrate and high active lignocellulases at about 120 h, i.e. CMCase 8.58 U/g, cellobiase 10.97 U/g, FPAase 2.21 U/g, xylanase 446.25 U/g, lassase 9.2 IU/g. In this study, the strategy is satisfactorily developed to construct the starter for high effective and low-cost production of lignocellulases using metropolis lignocellulosic waste defoliation as substrate, based on natural microflora and additional reinforcing with special strain.