Use of the GTPγS ([35S]GTPγS and Eu-GTPγS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors

被引：90
|
作者：
Strange, Philip G. [1 ]
机构：
[1] Univ Reading, Sch Pharm, Reading RG6 6UB, Berks, England
来源：
BRITISH JOURNAL OF PHARMACOLOGY | 2010年 / 161卷 / 06期
基金：
英国生物技术与生命科学研究理事会; 英国惠康基金;
关键词：
agonist; antagonist; inverse agonist; functional assay; GTP gamma S binding assay; efficacy; drug screening; SCINTILLATION PROXIMITY ASSAY; UNDERLYING AGONIST EFFICACY; HUMAN DOPAMINE-D-2 RECEPTOR; M-3 MUSCARINIC RECEPTORS; PHARMACOLOGICAL CHARACTERIZATION; CHO-CELLS; INVERSE AGONIST; RAT THALAMUS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); GUANINE-NUCLEOTIDES;
D O I：
10.1111/j.1476-5381.2010.00963.x
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
LINKED ARTICLES This article is part of a themed section on Analytical Receptor Pharmacology in Drug Discovery. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2010.161.issue-6.
引用
收藏
页码：1238 / 1249
页数：12
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