Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies

被引：10
|
作者：
Cheng, Ta-Chun [1 ]
Pan, Chu-Hsiang [2 ]
Chen, Chien-Shu [3 ]
Chuang, Kuo-Hsiang [1 ]
Chuang, Chih-Hung [4 ]
Huang, Chien-Chaio [4 ]
Chu, Yu-Yi [5 ]
Yang, Ya-Chun [4 ]
Chu, Pei-Yu [4 ]
Kao, Chien-Han [6 ]
Hsieh, Yuan-Chin [6 ]
Cheng, Tian-Lu [4 ,6 ,7 ]
机构：
[1] Taipei Med Univ, Grad Inst Pharmacognosy, Taipei 110, Taiwan
[2] Anim Hlth Res Inst, Council Agr, Div Hog Cholera Res, New Taipei City 251, Taiwan
[3] China Med Univ, Sch Pharm, Taichung 404, Taiwan
[4] Kaohsiung Med Univ, Dept Biomed & Environm Biol, Kaohsiung 807, Taiwan
[5] Natl Cheng Kung Univ, Coll Biosci & Biotechnol, Inst Bioinformat & Biosignal Transduct, Tainan 701, Taiwan
[6] Kaohsiung Med Univ, Grad Inst Med, Kaohsiung 807, Taiwan
[7] Kaohsiung Med Univ, Ctr Biomarkers & Biotech Drugs, Kaohsiung 807, Taiwan
来源：
VETERINARY JOURNAL | 2015年 / 205卷 / 01期
关键词：
Classical swine fever virus; E2; Glycosylation; Mammalian expression; Medium-direct-coating ELISA; GLYCOSYLATION; RESPONSES; VACCINES;
D O I：
10.1016/j.tvjl.2015.02.007
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment. The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90%) than a commercial CSFV antibody diagnostic kit (62%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：107 / 109
页数：3
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