Pro-angiogenic effect of PC-3 exosomes in endothelial cells in vitro

被引:14
|
作者
Prigol, Anne Natalie [2 ]
Rode, Michele Patricia [2 ]
Silva, Adny Henrique [2 ]
Cisilotto, Julia [2 ]
Creczynski-Pasa, Tania Beatriz [1 ,2 ]
机构
[1] Univ Fed Santa Catarina, Dept Pharmaceut Sci, BR-88040900 Florianopolis, SC, Brazil
[2] Univ Fed Santa Catarina, Postgrad Program Pharm, BR-88040900 Florianopolis, SC, Brazil
关键词
Prostate cancer; Castration resistance; Exosomes; Angiogenesis; miRNA; PROSTATE-CANCER CELLS; ADENOSINE-TRIPHOSPHATASE; EXTRACELLULAR VESICLES; ANDROGEN RECEPTOR; TUMOR-SUPPRESSOR; MICROVESICLES; FIBROBLASTS; METASTASIS; MICRORNAS; DIFFERENTIATION;
D O I
10.1016/j.cellsig.2021.110126
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The progression to a castration-resistant prostate cancer can occur after treatment with androgen deprivation therapy, resulting in poor prognosis and ineffective therapy response. Hormone dependence transition has been associated with increased tumor vascularization. Considering that exosomes are important components in communication between tumor cells and the microenvironment, we examined the angiogenic potential of exo-somes released from Pca cell lines with distinctive profiles of androgen response through exosomes isolation, microscopy and uptake, functional assays follow up by microarray, RT-qPCR and bioinformatics analysis. HUVEC cells treated with PC-3 exosomes (androgen independent) showed increased invasion and tube formation ability. In order to identify microRNAs (miRNAs) related to the angiogenic response, the characterization of exosomal miRNA profile was performed. As result we suggest that the miR-27a-3p could be involved in the pro-angiogenic effect of PC-3 exosomes.
引用
收藏
页数:11
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