RAPD fingerprinting for genotypic differentiation of S. aureus clinical isolates from camel (Camelus dromedarius)

被引：0
|
作者：
Solanki, S [1 ]
Kataria, AK [1 ]
Sharma, R [1 ]
Singh, G [1 ]
机构：
[1] Coll Vet & Anim Sci, Dept Vet Microbiol, Bikaner 334001, India
来源：
JOURNAL OF CAMEL PRACTICE AND RESEARCH | 2005年 / 12卷 / 01期
关键词：
camel; genotyping; RAPD; S; aureus;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Randomly amplified polymorphic DNA (RAPD) typing was performed on 21 S. aureus isolates from clinical wounds and abscesses in camel. Out of ten primers chosen for screening to know discrimination ability for S. aureus isolates three primers namely opc 1,2 and 10 showed good discriminatory index and generated 151 polymorphic bands with size ranging from 200-2500 base pairs. All the isolates were classified into two groups. Group 1 contained only one isolate while rest 20 were in group 2. The study indicated that there is much more genetic variation than studied earlier and RAPD can successfully be applied for differentiation of S. aureus strains.
引用
收藏
页码：17 / 20
页数：4
相关论文
共 50 条
  • [11] The Relationship between Ciprofloxacin Resistance and Genotypic Changes in S. aureus Ocular Isolates
    Afzal, Madeeha
    Vijay, Ajay Kumar
    Stapleton, Fiona
    Willcox, Mark
    PATHOGENS, 2022, 11 (11):
  • [12] Genotypic Characterization of Clinical Isolates of Staphylococcus aureus from Pakistan
    Khan, Saeed
    Marasa, Bernard S.
    Sung, Kidon
    Nawaz, Mohamed
    PATHOGENS, 2021, 10 (08):
  • [13] MOLECULAR IDENTIFICATION OF 20 Escherichia coli ISOLATES FROM DEAD NEONATAL CAMEL CALVES (Camelus dromedarius) IN THE UNITED ARAB EMIRATES
    Hassan, F. A.
    Wernery, U.
    Joseph, M.
    Anouassi, A.
    Mariena, K.
    Rangsun, P.
    JOURNAL OF CAMEL PRACTICE AND RESEARCH, 2019, 26 (03) : 259 - 260
  • [14] Phenotypic and genotypic characterization of clinical Staphylococcus aureus isolates from Kenya
    Kyany'a, Cecilia
    Nyasinga, Justin
    Matano, Daniel
    Oundo, Valerie
    Wacira, Simon
    Sang, Willie
    Musila, Lillian
    BMC MICROBIOLOGY, 2019, 19 (01)
  • [15] Phenotypic and genotypic characterization of clinical Staphylococcus aureus isolates from Kenya
    Cecilia Kyany’a
    Justin Nyasinga
    Daniel Matano
    Valerie Oundo
    Simon Wacira
    Willie Sang
    Lillian Musila
    BMC Microbiology, 19
  • [16] Genomics, phylogeny and in silico analysis of mitochondrial glutathione S-transferase-kappa from the camel Camelus dromedarius
    Ataya, Farid S.
    Al-Jafari, Abdul Aziz
    Daoud, Mohamed S.
    Al-Hazzani, Amal Abdulaziz
    Shehata, Afaf Ibrahim
    Saeed, Hesham M.
    Fouad, Dalia
    RESEARCH IN VETERINARY SCIENCE, 2014, 97 (01) : 46 - 54
  • [17] GENOTYPIC DIFFERENTIATION OF S. INTERMEDIUS AND S. PSEUDINTERMEDIUS STRAINS ISOLATED FROM DOGS AND CATS
    Bucur, Iulia
    Catana, N.
    Necsulescu, M.
    Imre, K.
    Herman, V
    Nichita, Ileana
    Tirziu, E.
    REVISTA ROMANA DE MEDICINA VETERINARA, 2020, 30 (03): : 81 - 84
  • [18] The gene contents and the antibiotic resistance phenotypes of the S. aureus clinical isolates
    Junie, Lia Monica
    Simon, L. M.
    Flonta, M.
    Almasanu, A.
    Homorodean, D.
    Ilie, R.
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2013, 32 : S64 - S64
  • [19] Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
    Tuchscherr, Lorena
    Poellath, Christine
    Siegmund, Anke
    Deinhardt-Emmer, Stefanie
    Hoerr, Verena
    Svensson, Carl-Magnus
    Figge, Marc Thilo
    Monecke, Stefan
    Loeffler, Bettina
    TOXINS, 2019, 11 (03):
  • [20] Genetic diversity of Fusarium oxysporum isolates from cucumber:: Differentiation by pathogenicity, vegetative compatibility, and RAPD fingerprinting
    Vakalounakis, DJ
    Fragkiadakis, GA
    PHYTOPATHOLOGY, 1999, 89 (02) : 161 - 168
12345