Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels

被引：1
|
作者：
Nagahara, Ayumi Inoue [1 ]
Homma, Jun [2 ]
Ryu, Bikei [3 ]
Sekine, Hidekazu [2 ]
Higashi, Yuhei [2 ,4 ]
Shimizu, Tatsuya [2 ]
Kawamata, Takakazu [3 ]
机构：
[1] Tokyo Womens Med Univ, Grad Sch, Dept Neurosurg, 8-1 Kawada Cho,Shinjuku Ku, Tokyo 1628666, Japan
[2] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, TWIns, 8-1 Kawada Cho,Shinjuku Ku, Tokyo 1628666, Japan
[3] Tokyo Womens Med Univ Hosp, Dept Neurosurg, 8-1 Kawada Cho,Shinjuku Ku, Tokyo 1628666, Japan
[4] Tokaihit Co Ltd, Shizuoka, Japan
来源：
SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期
基金：
日本学术振兴会;
关键词：
IN-VITRO; ENGINEERED BLOOD; VEGF-C; CAPILLARIES; VASCULATURE;
D O I：
10.1038/s41598-022-26041-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by co-culturing LECs and adipose-derived stem cells (ASCs) on temperature-responsive culture dishes. A cell ratio of 3:2 (vs. 1:4) generated networks with more branches and longer branch lengths. LEC-derived lymphatic vessels were observed 2 weeks after transplantation of a three-layered cell sheet construct onto rat gluteal muscle. Lymphatic vessel number, diameter and depth were greatest for a construct comprising two ASC sheets stacked on a LEC/ASC (3:2 ratio) sheet. Transplantation of this construct in a rat model of femoral lymphangiectomy led to the formation of functional lymphatic vessels containing both transplanted and host LECs. Further development of this technique may lead to a new method of promoting lymphangiogenesis.
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页数：16
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