Molecular cloning and disruption of a novel gene encoding UDP-glucose:tetrahydrobiopterin α-glucosyltransferase in the cyanobacterium Synechococcus sp PCC 7942

被引：15

作者：

Choi, YK ^{[1
]}

Hwang, YK ^{[1
]}

Park, YS ^{[1
]}

机构：

[1] Inje Univ, Dept Microbiol, Kimhae 621749, South Korea

来源：

FEBS LETTERS | 2001年 / 502卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

tetrahydrobiopterin-glucoside; glucosyltransferase; pteridine glycosyltransferase; gene disruption overexpression; cyanobacterium; Synechocystis sp PCC 6803;

D O I：

10.1016/S0014-5793(01)02667-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding UDP-glucose:tetrahydrobiopterin alpha -glucosyltransferase (BGluT) was cloned from the genomic DNA of Synechococcus sp. PCC 7942. The encoded protein consisting of 359 amino acid residues was verified in vitro and in vivo to be responsible for the synthesis of tetrahydrobiopterin (BH4)-glucoside produced in the organism. The BGluT gene is the first cloned in pteridine glycosyltransferases and also a novel one cloned so far in UDP-glycosyltransferases. The mutant cells disrupted in the BGluT gene produced only aglycosidic BH4 at a level of 8.3% of the BH4-glucoside in wild type cells and exhibited half of the wild type growth in normal photoautotrophic conditions. These results suggest that the glucosylation of BH4 is required for the maintenance of the high cellular concentration of the compound, thereby supporting the normal growth of Synechococcus sp. PCC 7942. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science BA. All rights reserved.

引用

页码：73 / 78

页数：6

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