Enteric viruses can be transmitted by mussels. Among me enteric viruses, the enteroviruses, the calicivirus and the hepatitis A virus represent an important public health problem. Enteric viruses and especially HAV are more resistant than bacteria to common sewage treatment and are able to survive for a long time in seawater. The AA studied a screening method of RT-nested-PCR for determining viral contamination of mussels. The experiments were carried out - using mussels collected from Adriatic Sea - to determine the following parameters: E. coli, enteroviruses and HAV. To confirm the infectivity of viruses the cell colture inoculation with positive samples was performed. The results show the presence of viral contamination in 41.7% of mussel samples and confirm the absence of correlation among E. coli and enteric viruses.
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Delaware State Univ, ARS, USDA, Microbial Food Safety Res Unit, Dover, DE 19901 USADelaware State Univ, ARS, USDA, Microbial Food Safety Res Unit, Dover, DE 19901 USA
Richards, Gary P.
McLeod, Catherine
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S Australian Res & Dev Inst, Adelaide, SA 5001, AustraliaDelaware State Univ, ARS, USDA, Microbial Food Safety Res Unit, Dover, DE 19901 USA
McLeod, Catherine
Le Guyader, Francoise S.
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IFREMER, Ctr Nantes, Microbiol Lab, F-44311 Nantes 03, FranceDelaware State Univ, ARS, USDA, Microbial Food Safety Res Unit, Dover, DE 19901 USA