A Novel Dimeric Conotoxin, FrXXA, from the Vermivorous Cone Snail Conus fergusoni, of the Eastern Pacific, Inhibits Nicotinic Acetylcholine Receptors

被引：1

作者：

Rodriguez-Ruiz, Ximena C. ^{[1
]}

Aguilar, Manuel B. ^{[2
]}

Ortiz-Arellano, Monica A. ^{[3
]}

Safavi-Hemami, Helena ^{[4
,5
]}

Lopez-Vera, Estuardo ^{[6
]}

机构：

[1] Univ Nacl Autonoma Mexico, Posgrad Ciencias Mar & Limnol, Mexico City 04510, DF, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Neurobiol, Lab Neurofarmacol Marina, Dept Neurobiol Celular & Mol, Queretaro 76230, Mexico

[3] Univ Autonoma Sinaloa, Fac Ciencias Mar, Lab Malacol, Mazatlan 82000, Mexico

[4] Univ Copenhagen, Dept Biomed Sci, DK-1172 Copenhagen, Denmark

[5] Univ Utah, Dept Biochem, Salt Lake City, UT 84112 USA

[6] Univ Nacl Autonoma Mexico, Lab Toxinol Marina, Unidad Acad Ecol & Biodiversidad Acuat, Inst Ciencias Mar & Limnol, Mexico City 04510, DF, Mexico

来源：

TOXINS | 2022年 / 14卷 / 08期

基金：

欧盟地平线“2020”;

关键词：

alpha d-conotoxin; dimeric-peptides; nAChR; Conus snails; Conus fergusoni; PEPTIDES; BIODIVERSITY; CONOPEPTIDES; STRATEGY;

D O I：

10.3390/toxins14080510

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

We isolated a new dimeric conotoxin with inhibitory activity against neuronal nicotinic acetylcholine receptors. Edman degradation and transcriptomic studies indicate a homodimeric conotoxin composed by two chains of 47 amino acid in length. It has the cysteine framework XX and 10 disulfide bonds. According to conotoxin nomenclature, it has been named as alpha D-FrXXA. The alpha D-FrXXA conotoxin inhibited the ACh-induced response on nAChR with a IC50 of 125 nM on h alpha 7, 282 nM on h alpha 3 beta 2, 607 nM on alpha 4 beta 2, 351 nM on mouse adult muscle, and 447 nM on mouse fetal muscle. This is first toxin characterized from C. fergusoni and, at the same time, the second alpha D-conotoxin characterized from a species of the Eastern Pacific.

引用

页数：13

共 25 条

[21] A Novel Lid-Covering Peptide Inhibitor of Nicotinic Acetylcholine Receptors Derived from D-Conotoxin GeXXA
Yang, Longjin
Tae, Han-Shen
Fan, Zhou
Shao, Xiaoxia
Xu, Shaoqiong
Zhao, Suwen
Adams, David J.
Wang, Chunguang
MARINE DRUGS, 2017, 15 (06):
[22] STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF A NOVEL α-CONOTOXIN BT1.8 FROM CONUS BETULINUS SELECTIVELY TARGETING NEURONAL α6/α3β2β3 AND α3β2 NICOTINIC ACETYLCHOLINE RECEPTORS
Ning, Huying
Huang, Biling
Liu, Zhuguo
Li, Liang
Zhang, Longxiao
Guo, Chenyun
Dai, Qiuyun
TOXICON, 2019, 158 : S68 - S68
[23] Mass spectrometric identification and denovo sequencing of novel conotoxins from vermivorous cone snail (Conus inscriptus), and preliminary screening of its venom for biological activities in vitro and in vivo
Jain, Ruchi P.
Jayaseelan, Benjamin Franklin
Alphonse, Carlton Ranjith Wilson
Mahmoud, Ahmed Hossam
Mohammed, Osama B.
Almunqedhi, Bandar Mohsen Ahmed
Pushpabai, Rajesh Rajaian
SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2021, 28 (03) : 1582 - 1595
[24] Novel conorfamides from Conus austini venom modulate both nicotinic acetylcholine receptors and acid-sensing ion channels
Jin, Ai-hua
Cristofori-Armstrong, Ben
Rash, Lachlan D.
Roman-Gonzalez, Sergio Agustin
Arreguin Espinosa, Roberto
Lewis, Richard J.
Alewood, Paul F.
Vetter, Irina
BIOCHEMICAL PHARMACOLOGY, 2019, 164 : 342 - 348
[25] Identification, by RT-PCR, of eight novel I2-conotoxins from the worm-hunting cone snails Conus brunneus, Conus nux, and Conus princeps from the eastern Pacific (Mexico)
Zamora-Bustillos, R.
Rivera-Reyes, R.
Aguilar, M. B.
Michel-Morfin, E.
Landa-Jaime, V.
Falcon, A.
Heimer, E. P.
PEPTIDES, 2014, 53 : 22 - 29

← 1 2 3 →