Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing

被引：17
|
作者：
Willoughby, Kim [1 ]
Thomson, Karen [1 ]
Maley, Madeleine [1 ]
Gilray, Janice [1 ]
Scholes, Sandra [2 ]
Howie, Fiona [3 ]
Caldow, George [4 ]
Nettleton, Peter F. [1 ]
机构：
[1] Int Res Ctr, Moredun Res Inst, Penicuik EH26 0PZ, Midlothian, Scotland
[2] Int Res Ctr, Vet Labs Agcy Lasswade, Penicuik EH26 0PZ, Midlothian, Scotland
[3] Scottish Agr Coll, Edinburgh Dis Surveillance Ctr, Penicuik EH26 0QE, Midlothian, Scotland
[4] Scottish Agr Coll, St Boswells Dis Surveillance Ctr Greycrook, St Boswells TD6 0EU, Roxburghshire, Scotland
来源：
VETERINARY MICROBIOLOGY | 2008年 / 126卷 / 1-3期
关键词：
bovine respiratory syncytial virus (BRSV); diagnosis; real time RT-PCR; immunohistochemistry; immunofluorescence;
D O I：
10.1016/j.vetmic.2007.07.005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：264 / 270
页数：7
相关论文
共 50 条
  • [31] Detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the Beaudette strain
    Jackwood, MW
    Hilt, DA
    Callison, SA
    [J]. AVIAN DISEASES, 2003, 47 (03) : 718 - 724
  • [32] Comparison of virus isolation and reverse transcription polymerase chain reaction assay for detection of bovine viral diarrhea virus in bulk milk tank samples
    Renshaw, RW
    Ray, R
    Dubovi, EJ
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2000, 12 (02) : 184 - 186
  • [33] Optimisation and validation of a real-time reverse transcriptase-polymerase chain reaction assay for detection of betanodavirus
    Hick, Paul
    Whittington, Richard J.
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2010, 163 (02) : 368 - 377
  • [34] Comparison of immunochromatographic diagnostic test with heminested reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
    Sharma, Pranoti
    Singh, C. K.
    Narang, Deepti
    [J]. VETERINARY WORLD, 2015, 8 (02) : 135 - 138
  • [35] Utility of real time polymerase chain reaction in the diagnosis of respiratory syncytial virus infection among adult patients
    Rabagliati B., Ricardo
    Serri V., Michel
    Montecinos P, Luisa
    Azocar A., Teresa
    Ferres G., Marcela
    [J]. REVISTA CHILENA DE INFECTOLOGIA, 2007, 24 (06): : 441 - 445
  • [36] Reverse transcription-polymerase chain reaction amplification of respiratory syncytial virus genome from neonatal nasal swab samples
    Yoshio, H
    Yamada, M
    Nii, S
    [J]. ACTA PAEDIATRICA JAPONICA, 1996, 38 (05): : 429 - 433
  • [37] Development and application of reverse transcriptase nested polymerase chain reaction test for the detection of exogenous avian leukosis virus
    García, M
    El-Attrache, J
    Riblet, SM
    Lunge, VR
    Fonseca, ASK
    Villegas, P
    Ikuta, N
    [J]. AVIAN DISEASES, 2003, 47 (01) : 41 - 53
  • [38] Detection of Human Metapneumovirus and Respiratory Syncytial Virus by Real-Time Polymerase Chain Reaction Among Hospitalized Young Children in Iran
    Parsania, Masoud
    Poopak, Behzad
    Pouriayevali, Mohammad Hassan
    Haghighi, Sama
    Amirkhani, Aref
    Nateghian, Alireza
    [J]. JUNDISHAPUR JOURNAL OF MICROBIOLOGY, 2016, 9 (03)
  • [39] Evaluation of a multiplex real-time polymerase chain reaction assay for the detection of influenza and respiratory syncytial viruses
    Esposito, Susanna
    Scala, Alessia
    Tagliabue, Claudia
    Zampiero, Alberto
    Bianchini, Sonia
    Principi, Nicola
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2016, 84 (01) : 40 - 42
  • [40] Comparison of a conventional polymerase chain reaction with real-time polymerase chain reaction for the detection of neurotropic viruses in cerebrospinal fluid samples
    Ramamurthy, M.
    Alexander, M.
    Aaron, S.
    Kannangai, R.
    Ravi, V.
    Sridharan, G.
    Abraham, A. M.
    [J]. INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 29 (02) : 102 - 109
12345