Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing

被引：17
|
作者：
Willoughby, Kim [1 ]
Thomson, Karen [1 ]
Maley, Madeleine [1 ]
Gilray, Janice [1 ]
Scholes, Sandra [2 ]
Howie, Fiona [3 ]
Caldow, George [4 ]
Nettleton, Peter F. [1 ]
机构：
[1] Int Res Ctr, Moredun Res Inst, Penicuik EH26 0PZ, Midlothian, Scotland
[2] Int Res Ctr, Vet Labs Agcy Lasswade, Penicuik EH26 0PZ, Midlothian, Scotland
[3] Scottish Agr Coll, Edinburgh Dis Surveillance Ctr, Penicuik EH26 0QE, Midlothian, Scotland
[4] Scottish Agr Coll, St Boswells Dis Surveillance Ctr Greycrook, St Boswells TD6 0EU, Roxburghshire, Scotland
来源：
VETERINARY MICROBIOLOGY | 2008年 / 126卷 / 1-3期
关键词：
bovine respiratory syncytial virus (BRSV); diagnosis; real time RT-PCR; immunohistochemistry; immunofluorescence;
D O I：
10.1016/j.vetmic.2007.07.005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：264 / 270
页数：7
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