Crystallization and preliminary X-ray characterization of a lectin from Cicer arietinum (chickpea)

被引：20
|
作者：
Katre, UV
Gaikwad, SM
Bhagyawant, SS
Deshpande, UD
Khan, MI
Suresh, CG [1 ]
机构：
[1] Natl Chem Lab, Div Biochem Sci, Pune 411008, Maharashtra, India
[2] SRTM Univ, Sch Life Sci, Nanded 431606, India
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷
关键词：
D O I：
10.1107/S1744309104032166
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The lectin isolated from mature seeds of Cicer arietinum (CAL) agglutinates pronase-treated rabbit and human erythrocytes and its haemagglutination activity is inhibited by fetuin and desialated fetuin but not by simple monosaccharides or oligosaccharides. The purified lectin is a dimer of molecular weight 43 000 Da composed of two identical subunits ( MW 21 500), as confirmed by SDS PAGE. The lectin has been crystallized using the hanging-drop vapour-diffusion method at 295 K over a well solution containing 0.2 M sodium acetate, 0.1 M sodium phosphate buffer pH 6.5 and 14%(w/v) polyethylene glycol 8000. The triangular prism-shaped crystals belong to space group R3 and have unit-cell parameters a = b = 81.2, c = 69.4 angstrom. The diffraction data are 93.8% complete to 2.3 angstrom Bragg spacing with an R-merge of 0.103.
引用
收藏
页码：141 / 143
页数：3
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