Comparison of an immunochromatographic rapid test with enzyme-linked immunosorbent assay and polymerase chain reaction for the detection of Shiga toxins from human stool samples

被引:7
|
作者
Grif, Katharina [1 ]
Orth, Dorothea
Dierich, Manfred P.
Wuerzner, Reinhard
机构
[1] Innsbruck Med Univ, Dept Hyg Microbiol & Social Med, A-6020 Innsbruck, Austria
[2] Austrian Reference Ctr Enterohemorrhag Escherichi, A-6020 Innsbruck, Austria
关键词
EHEC; Duopath Verotoxin-test (TM); ELISA; PCR;
D O I
10.1016/j.diagmicrobio.2007.03.022
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Rapid detection of enterohemorrhagic Escherichia coli is important for its successful treatment. We have evaluated the immunochromatographic Duopath Verotoxin-test (TM) for detection of Shiga toxins, in comparison with enzyme-linked immunosorbent assay and polymerase chain reaction, on 240 clinical human stool samples. The Duopath-test showed a lower sensitivity and specificity. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:97 / 99
页数:3
相关论文
共 50 条
  • [21] Detection of egg drop syndrome virus antigen or genome by enzyme-linked immunosorbent assay or polymerase chain reaction
    Raj, GD
    Sivakumar, S
    Matheswaran, K
    Chandrasekhar, M
    Thiagarajan, V
    Nachimuthu, K
    [J]. AVIAN PATHOLOGY, 2003, 32 (05) : 545 - 550
  • [22] Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip for Rapid Detection of Atrazine in Three Medicinal Herbal Roots
    Yu-Dan Wang
    Jia-An Qin
    Liu Wu
    Bao-Min Wang
    Sergei Eremin
    Shi-Hai Yang
    Mei-Hua Yang
    [J]. World Journal of Traditional Chinese Medicine, 2021, 7 (01) : 97 - 103
  • [23] Enzyme-linked immunosorbent assay and immunochromatographic strip for rapid detection of atrazine in three medicinal herbal roots
    Wang, Yu-Dan
    Qin, Jia-An
    Wu, Liu
    Wang, Bao-Min
    Eremin, Sergei
    Yang, Shi-Hai
    Yang, Mei-Hua
    [J]. WORLD JOURNAL OF TRADITIONAL CHINESE MEDICINE, 2021, 7 (01) : 97 - 103
  • [24] DETECTION OF HELICOBACTER PYLORI ANTIGEN IN STOOL BY ENZYME-LINKED IMMUNOSORBENT ASSAY AND COMPARISON WITH CONVENTIONAL METHODS
    Selvi, Rajesh Kumar Rajkumar
    Shankeregowda, Suraj Holalu
    Yuvaraja
    Subburaj
    [J]. JOURNAL OF EVOLUTION OF MEDICAL AND DENTAL SCIENCES-JEMDS, 2016, 5 (51): : 3408 - 3411
  • [25] Monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic rapid assay for dihydrostreptomycin in milk
    Watanabe, H
    Satake, A
    Kido, Y
    Tsuji, A
    [J]. ANALYTICA CHIMICA ACTA, 2002, 472 (1-2) : 45 - 53
  • [26] LcrV capture enzyme-linked immunosorbent assay for detection of Yersinia pestis from human samples
    Gomes-Solecki, MJC
    Savitt, AG
    Rowehl, R
    Glass, JD
    Bliska, JB
    Dattwyler, RJ
    [J]. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2005, 12 (02) : 339 - 346
  • [27] Comparison of blood polymerase chain reaction and enzyme-linked immunosorbent assay for detection of Mycobacterium avium subsp parataberculosis infection in cattle and sheep
    Juste, RA
    Garrido, JM
    Geijo, M
    Elguezabal, N
    Aduriz, G
    Atxaerandio, R
    Sevilla, I
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2005, 17 (04) : 354 - 359
  • [28] Comparison of Real-Time Polymerase Chain Reaction and Enzyme-Linked Immunosorbent Assay for Sensitive and Quantitative Detection of Hazelnuts in Nut Pastes
    Piknova, L'ubica
    Janska, Veronika
    Kuchta, Tomas
    Siekel, Peter
    [J]. JOURNAL OF AOAC INTERNATIONAL, 2018, 101 (06) : 1864 - 1867
  • [29] Rapid enzyme-linked immunosorbent assay and immunochromatographic strip for detecting ribavirin in chicken muscles
    Peng, Shuang
    Song, Shanshan
    Liu, Liqiang
    Kuang, Hua
    Xu, Chuanlai
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2016, 27 (04) : 449 - 459
  • [30] Comparison of colorimetric and chemiluminescent enzyme-linked immunosorbent assay for the detection of endosulfan in food samples
    Deepak, T. S.
    Rashmi, Shenoy
    Manonmani, Haravey K.
    [J]. LUMINESCENCE, 2015, 30 (08) : 1274 - 1279