Development of Molecular Tools for Characterization and Genetic Diversity Analysis in Tunisian Fig (Ficus carica) Cultivars

被引：17

作者：

Chatti, Khaled ^{[1
,3
]}

Baraket, Ghada ^{[1
]}

Abdelkrim, Ahmed Ben ^{[1
]}

Saddoud, Olfa ^{[1
]}

Mars, Messaoud ^{[2
]}

Trifi, Mokhtar ^{[1
]}

Hannachi, Amel Salhi ^{[1
]}

机构：

[1] Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Tunis 2092, Tunisia

[2] Inst Super Agron, Sousse 4042, Tunisia

[3] Fac Sci Gafsa, Gafsa 2112, Tunisia

来源：

BIOCHEMICAL GENETICS | 2010年 / 48卷 / 9-10期

关键词：

Ficus carica L; ISSR; RAMPO; RAPD; SSR; L; RAPD; RELATEDNESS; GENOTYPES; MARKERS; ISSR;

D O I：

10.1007/s10528-010-9360-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on the combined data show considerable genetic diversity, and the tested markers discriminated all fig genotypes studied. The identification key established on the basis of SSR permitted the unambiguous discrimination of cultivars and confirmed the reliability of SSR for fingerprinting fig genotypes. The study findings are discussed in relation to the establishment of a national reference collection that will aid in the conservation of Tunisian fig resources.

引用

页码：789 / 806

页数：18

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