Specific detection of RNA on ultra-thin sections

被引：3
|
作者：
Calabuig, J
de Juan, J
Iborra, FJ [1 ]
机构：
[1] John Radcliffe Hosp, Weatherall Inst Mol Med, MRC, Mol Haematol Unit, Oxford OX3 9DS, England
[2] Inst Canc Res, INSTRACAN, Alicante 03400, Spain
[3] Univ Alicante, Fac Ciencias, Dept Biotecnol, E-03080 Alicante, Spain
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 2005年 / 152卷 / 02期
基金：
英国医学研究理事会;
关键词：
electron microscopy; nucleic acid detection; RNA;
D O I：
10.1016/j.jsb.2005.06.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this paper, we describe a specific method for ultrastructural detection of RNA. Our method is based in the bromination "in situ" of uridine residues in the RNA, which allows the detection of brominated RNA with specific antibodies against bromo-deoxyuridine. With this method we can achieve high specificity and resolution, and it can be applied to Epon or acrylic resin embedded material. (C) 2005 Published by Elsevier Inc.
引用
收藏
页码：146 / 148
页数：3
相关论文
共 50 条
  • [21] ELECTRON MICROSCOPIC OBSERVATIONS OF STRUCTURE IN ULTRA-THIN SECTIONS OF VITRINITE IN COAL
    MCCARTNEY, J
    WALLINE, RE
    ERGUN, S
    NATURE, 1961, 191 (479) : 1361 - &
  • [22] Elastic volume reconstruction from series of ultra-thin microscopy sections
    Saalfeld, Stephan
    Fetter, Richard
    Cardona, Albert
    Tomancak, Pavel
    NATURE METHODS, 2012, 9 (07) : 717 - U280
  • [23] PREPARATION OF ULTRA-THIN FROZEN SECTIONS OF PLANT TISSUES FOR ELECTRON MICROSCOPY
    GAHAN, PB
    GREENOAK, GC
    JAMES, D
    HISTOCHEMIE, 1970, 24 (03): : 230 - +
  • [24] Elastic volume reconstruction from series of ultra-thin microscopy sections
    Saalfeld S.
    Fetter R.
    Cardona A.
    Tomancak P.
    Nature Methods, 2012, 9 (7) : 717 - 720
  • [25] Detection and characterization of ultra-thin films with neutron reflectometry
    Tun, Z.
    ACTA CRYSTALLOGRAPHICA SECTION A, 2009, 65 : 1 - 4
  • [26] DETECTION OF GLOMERULAR ANIONIC SITES IN POST-EMBEDDED ULTRA-THIN SECTIONS USING CATIONIC COLLOIDAL GOLD
    GOODE, NP
    SHIRES, M
    CRELLIN, DM
    DAVISON, AM
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1991, 39 (07) : 965 - 972
  • [27] These batteries are ultra-thin
    不详
    R&D MAGAZINE, 1996, 38 (10): : 66 - 66
  • [28] ULTRA-THIN DSAEK
    Beltz, Jacqueline
    Busin, Massimo
    CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2012, 40 : 68 - 69
  • [29] Specific response of ultra-thin metal oxide films to gas
    Setkus, Arunas
    Bukauskas, Virginijus
    Mironas, Audruzis
    Senuliene, Daiva
    Strazdiene, Viktorija
    PHYSICA STATUS SOLIDI C - CURRENT TOPICS IN SOLID STATE PHYSICS, VOL 6, NO 12, 2009, 6 (12): : 2753 - 2755
  • [30] Ultra-thin MobileNet
    Sinha, Debjyoti
    El-Sharkawy, Mohamed
    2020 10TH ANNUAL COMPUTING AND COMMUNICATION WORKSHOP AND CONFERENCE (CCWC), 2020, : 234 - 240
12345