Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells

被引：12
|
作者：
She, Zhen-Yu [1 ,2 ]
Yu, Kai-Wei [1 ,2 ]
Zhong, Ning [1 ,2 ]
Xiao, Yu [1 ,2 ]
Wei, Ya-Lan [3 ,4 ]
Lin, Yang [1 ,2 ]
Li, Yue-Ling [1 ,2 ]
Lu, Ming-Hui [1 ,2 ]
机构：
[1] Fujian Med Univ, Dept Cell Biol & Genet, Sch Basic Med Sci, Fuzhou 350122, Fujian, Peoples R China
[2] Fujian Prov Univ, Key Lab Stem Cell Engn & Regenerat Med, Fuzhou 350122, Fujian, Peoples R China
[3] Fujian Obstet & Gynecol Hosp, Fuzhou 350001, Fujian, Peoples R China
[4] Fujian Med Univ, Med Res Ctr, Fujian Prov Childrens Hosp, Fujian Matern & Child Hlth Hosp,Affiliated Hosp, Fuzhou 350001, Fujian, Peoples R China
来源：
CELL DEATH DISCOVERY | 2020年 / 6卷 / 01期
关键词：
PUTATIVE KINETOCHORE MOTOR; MITOTIC CHECKPOINT; PROTEIN-E; MICROTUBULE ATTACHMENT; METAPHASE; ANEUPLOIDY; DOMAIN; BUBR1; END; MISSEGREGATION;
D O I：
10.1038/s41420-020-0261-8
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Kinesin-7 CENP-E is an essential kinetochore motor required for chromosome alignment and congression. However, the specific functions of CENP-E in the spermatogenic cells during spermatogenesis remain unknown. In this study, we find that CENP-E proteins are expressed in the spermatogonia, spermatocytes, and the elongating spermatids. CENP-E inhibition by specific inhibitor GSK923295 results in the disruption of spermatogenesis and cell cycle arrest of spermatogenic cells. Both spermatogonia and spermatocytes are arrested in metaphase and several chromosomes are not aligned at the equatorial plate. We find that CENP-E inhibition leads to chromosome misalignment, the spindle disorganization, and the formation of the aneuploidy cells. Furthermore, the inhibition of CENP-E results in the defects in the formation of spermatids, including the sperm head condensation and the sperm tail formation. We have revealed that kinesin-7 CENP-E is essential for chromosome alignment and genome stability of the spermatogenic cells.
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页数：17
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