MicroED for the study of protein-ligand interactions and the potential for drug discovery

被引：7

作者：

Clark, Lisa J. ^{[1
]}

Bu, Guanhong ^{[2
,3
]}

Nannenga, Brent L. ^{[2
,3
]}

Gonen, Tamir ^{[1
,4
,5
]}

机构：

[1] Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90024 USA

[2] Arizona State Univ, Sch Engn Matter Transport & Energy, Chem Engn, Tempe, AZ 85287 USA

[3] Arizona State Univ, Ctr Appl Struct Discovery Biodesign Inst, Tempe, AZ 85287 USA

[4] Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90024 USA

[5] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA

来源：

NATURE REVIEWS CHEMISTRY | 2021年 / 5卷 / 12期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

ELECTRON-DIFFRACTION DATA; DATA-COLLECTION; CRYSTAL-STRUCTURE; SCATTERING FACTORS; TOMOGRAPHY; CRYSTALLOGRAPHY; MICROCRYSTALS; NANOCRYSTALS; REFINEMENT; MEMBRANE;

D O I：

10.1038/s41570-021-00332-y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Microcrystal electron diffraction (MicroED) is an electron cryo-microscopy (cryo-EM) technique used to determine molecular structures with crystals that are a millionth the size needed for traditional single-crystal X-ray crystallography. An exciting use of MicroED is in drug discovery and development, where it can be applied to the study of proteins and small molecule interactions, and for structure determination of natural products. The structures are then used for rational drug design and optimization. In this Perspective, we discuss the current applications of MicroED for structure determination of protein-ligand complexes and potential future applications in drug discovery.

引用

页码：853 / 858

页数：6

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