Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG

被引：10

作者：

van Eeden, Pauline E. ^{[1
]}

Wiese, Michael D. ^{[2
,3
]}

Aulfrey, Susan ^{[1
]}

Hales, Belinda J. ^{[4
]}

Stone, Shelley F. ^{[1
]}

Brown, Simon G. A. ^{[1
,3
,5
]}

机构：

[1] Univ Western Australia, Ctr Clin Res Emergency Med, Western Australian Inst Med Res, Perth, WA 6009, Australia

[2] Univ S Australia, Samson Inst Hlth Res, Adelaide, SA 5001, Australia

[3] Royal Hobart Hosp, Dept Med, Hobart, Tas, Australia

[4] Univ Western Australia, Ctr Child Hlth Res, Telethon Inst Child Hlth Res, Perth, Australia

[5] Royal Perth Hosp, Dept Emergency Med, Perth, WA, Australia

来源：

PLOS ONE | 2011年 / 6卷 / 01期

基金：

英国医学研究理事会;

关键词：

JUMPER ANT VENOM; ALLERGY; DIAGNOSIS; PREVALENCE; ANTIBODIES; HISTAMINE; SEVERITY; RELEASE; HISTORY; DISEASE;

D O I：

10.1371/journal.pone.0016741

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We adapted DELFIA (TM) (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide- specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.

引用

页数：7

共 50 条

[31] Differences in venom-specific IgE in symptomatic and asymptomatic subjects sensitized to Hymenoptera venoms
Bilò, MB
Incorvaia, C
Mauro, M
Cinti, B
Bonifazi, F
ALLERGY, 2002, 57 : 121 - 121
[32] Determinants of venom-specific IgE antibody concentration during long-term wasp venom immunotherapy
Pravettoni V.
Piantanida M.
Primavesi L.
Forti S.
Pastorello E.A.
Clinical and Molecular Allergy, 13 (1)
[33] TRIIODOTHYRONINE UPTAKE MEASUREMENT IN SERUM BY TIME-RESOLVED FLUORESCENCE IMMUNOFLUOROMETRY
TAN, YK
BHAYANA, V
KHOSRAVI, MJ
CLINICAL CHEMISTRY, 1990, 36 (06) : 1077 - 1077
[34] TRIIODOTHYRONINE UPTAKE MEASUREMENT IN SERUM BY TIME-RESOLVED FLUORESCENCE IMMUNOFLUOROMETRY
TAN, YK
BHAYANA, V
PAPANASTASIOUDIAMANDI, A
KHOSRAVI, MJ
JOURNAL OF IMMUNOASSAY, 1990, 11 (02): : 123 - 138
[35] FURTHER-STUDIES OF PATIENTS WITH BOTH HONEYBEE AND YELLOW JACKET VENOM-SPECIFIC IGE
REISMAN, RE
LAZELL, MI
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1985, 75 (01) : 207 - 207
[36] Time-resolved fluorescence spectroscopy
Millar, DP
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1996, 6 (05) : 637 - 642
[37] TIME-RESOLVED FLUORESCENCE OF PROTEINS
BEECHEM, JM
BRAND, L
ANNUAL REVIEW OF BIOCHEMISTRY, 1985, 54 : 43 - 71
[38] TIME-RESOLVED FLUORESCENCE SPECTROSCOPY
HOLZWARTH, AR
BIOCHEMICAL SPECTROSCOPY, 1995, 246 : 334 - 362
[39] Time-resolved fluorescence microscopy
Suhling, Klaus
Tregidgo, Carolyn
Sergent, Nicolas
Levitt, James A.
Pavlides, Alex
Green, Mark
Hungerford, Graham
Rei, Ana
Ferreira, M. Isabel C.
ADVANCED PHOTON COUNTING TECHNIQUES II, 2007, 6771
[40] Fluorescence time-resolved macroimaging
Shcheslavskiy, Vladislav, I
Shirmanova, Marina, V
Dudenkova, Varvara V.
Lukyanov, Konstantin A.
Gavrina, Alena, I
Shumilova, Anastasia, V
Zagaynova, Elena
Becker, Wolfgang
OPTICS LETTERS, 2018, 43 (13) : 3152 - 3155

← 1 2 3 4 5 →