Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG

被引：10
|
作者：
van Eeden, Pauline E. [1 ]
Wiese, Michael D. [2 ,3 ]
Aulfrey, Susan [1 ]
Hales, Belinda J. [4 ]
Stone, Shelley F. [1 ]
Brown, Simon G. A. [1 ,3 ,5 ]
机构：
[1] Univ Western Australia, Ctr Clin Res Emergency Med, Western Australian Inst Med Res, Perth, WA 6009, Australia
[2] Univ S Australia, Samson Inst Hlth Res, Adelaide, SA 5001, Australia
[3] Royal Hobart Hosp, Dept Med, Hobart, Tas, Australia
[4] Univ Western Australia, Ctr Child Hlth Res, Telethon Inst Child Hlth Res, Perth, Australia
[5] Royal Perth Hosp, Dept Emergency Med, Perth, WA, Australia
来源：
PLOS ONE | 2011年 / 6卷 / 01期
基金：
英国医学研究理事会;
关键词：
JUMPER ANT VENOM; ALLERGY; DIAGNOSIS; PREVALENCE; ANTIBODIES; HISTAMINE; SEVERITY; RELEASE; HISTORY; DISEASE;
D O I：
10.1371/journal.pone.0016741
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We adapted DELFIA (TM) (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide- specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.
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页数：7
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