Effects of β-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and protein structure

被引：3
|
作者：
Qi Zhitao [1 ]
Zhang Qihuan [1 ]
Dai Honglian [2 ]
Shao Rong [1 ]
Wang Aimin [1 ]
Li Shipu [2 ]
机构：
[1] Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224051, Peoples R China
[2] Wuhan Univ Technol, Biomat Res & Engn Ctr, Wuhan 430070, Peoples R China
来源：
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION | 2011年 / 26卷 / 06期
关键词：
beta-TCP ceramics; mineralization; osteoblast; Ca2+](i); protein structure; TRICALCIUM PHOSPHATE; LONG-TERM; BONE; HYDROXYAPATITE; CELLS;
D O I：
10.1007/s11595-011-0363-5
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
beta-TCP, as one of calcium phosphates ceramics, exerts perfect biocompatibility and osteoconductivity, and is clinically used as a bone graft substitute for decades. Consequently, the effects of beta-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and BSA protein structure were studied. Results showed that beta-TCP could increase the intracelluar Ca2+ concentration and mineralization of osteoblast, indicating that beta-TCP ceramics could take part in the organic metabolism and the degradation product had no detrimental effect on osteoblast in vitro. Furthermore, beta-TCP ceramics could increase the content of alpha-helix and beta-pleated sheet and change BSA into more ordering structure, those changes might be favorable for the biomineralization after beta-TCP ceramics implanted.
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页码：1064 / 1067
页数：4
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