The relationship between leadership and organizational culture is a main problem, which was studied by many scholars. Transactional leadership and transformational leadership have their own characteristics, which are in line with different organizational cultures. Through the research on the concrete evidences, this paper drew a conclusion, that is in rules orientation organizational culture, leaders are inclined to adopt transactional leadership which compared with transformational leadership can lead to subordinates' higher leader satisfaction, organizational commitment and job satisfaction. In innovation orientation and support orientation organizational culture, leaders prefer transformational leadership, which is more effective than transactional leadership.