Characterization of the pyrethroid resistance mechanisms in a Blattella germanica (Dictyoptera: Blattellidae) strain from Buenos Aires (Argentina)

被引:2
|
作者
Bone, Emiliano [1 ,2 ]
Roca Acevedo, Gonzalo [2 ,3 ]
Sterkel, Marcos [2 ,4 ]
Ons, Sheila [2 ,4 ]
Gonzalez-Audino, Paola [2 ,3 ]
Sfara, Valeria [1 ,2 ]
机构
[1] Univ Nacl San Martin, Inst Invest & Ingn Ambiental IIIA, CONICET, UNSAM, San Martin, Argentina
[2] Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina
[3] Consejo Nacl Invest Cient & Tecn, Ctr Invest Plagas & Insecticidas, CIPEIN, UNIDEF,CITEDEF, Buenos Aires, DF, Argentina
[4] Univ Nacl La Plata, Fac Ciencias Exactas, Ctr Reg Estudios Genom, Lab Neurobiol Insectos, Buenos Aires, DF, Argentina
关键词
Enzymatic activity; German cockroach; knockdown resistance; pyrethroids; TRIATOMA-INFESTANS HEMIPTERA; SODIUM-CHANNEL GENE; FIELD-COLLECTED STRAINS; MIXED-FUNCTION OXIDASE; INSECTICIDE RESISTANCE; COCKROACH DICTYOPTERA; KNOCKDOWN-RESISTANCE; SUSCEPTIBLE STRAINS; ION CHANNELS; KDR;
D O I
10.1017/S000748532100050X
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The use of chemical insecticides is the main control method for Blattella germanica worldwide. The prolonged and frequent use of insecticides produced the selection of insecticide-resistant individuals. The German cockroach is one of the most widespread urban pests in Argentina. In the last decades, resistance monitoring studies in this country demonstrated that there is a high prevalence of pyrethroid-resistant populations of B. germanica in the field. In this work, we studied the resistance mechanisms of a field-collected strain of B. germanica at toxicological, enzymatic, and molecular levels. A resistance ratio of 100 was obtained for the resistant strain when it was exposed to beta-cypermethrin. The pretreatment with specific synergists (piperonyl butoxide and triphenyl phosphate) led to a significant increase in the toxicity of the pyrethroid, suggesting an involvement of oxidases and esterases in the detoxification of this insecticide. Moreover, esterase and oxidase activities in the resistant strain were 1.5-fold and 2-fold higher respectively, compared to the susceptible individuals. On the other hand, the voltage-gated sodium channel gene of the resistant cockroaches did not show nucleotidic substitutions in the domain II which are associated to knockdown resistance in this species. These results suggest that the main mechanism of resistance of the studied cockroaches' strain is metabolic, mainly due to an increase in the activity of oxidase and esterase enzymes. The results of this work in addition to other reports found in literature show that the extended use of a single active principle for cockroach control promotes the development of resistance leading to control failure in the field. In contrast, integrated pest management strategies include the use of different control tools in addition to chemical insecticides, which delay the appearance of resistance increasing the efficacy of pest control.
引用
收藏
页码:21 / 28
页数:8
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