Maximization of Loading and Stability of ssDNA:Iron Oxide Nanoparticle Complexes Formed through Electrostatic Interaction

被引:16
|
作者
Berti, Lorenzo [1 ]
Woldeyesus, Temesgen [1 ]
Li, Yuanpei [1 ]
Lam, Kit S. [1 ]
机构
[1] Univ Calif Davis, Dept Biochem & Mol Med, Sacramento, CA 95817 USA
关键词
CELL PENETRATING PEPTIDES; ANTISENSE OLIGONUCLEOTIDES; GOLD NANOPARTICLES; GENE-THERAPY; SURFACE-PROPERTIES; SIRNA DELIVERY; CANCER; RIBOZYMES; NANOCRYSTALS; MECHANISMS;
D O I
10.1021/la103237e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The use of inorganic nanoparticles (NPs) as vectors for the delivery of oligonucleotides for in vitro and in vivo applications is rapidly gaining momentum. Some of the reasons making them especially good candidates for this purpose are their ease of synthesis in a range of sizes and surface coatings, their propensity to penetrate cell membranes, their stability and biocompatibility, and their unique size-dependent physical properties that impart additional diagnostic and therapeutic tools. Notwithstanding these notable attributes, a major obstacle to their practical use is given by the typically low oligonucleotide loading levels attainable through conventional bioconjugation procedures. This shortcoming is especially worrisome as toxicity concerns have been associated with codelivery of NPs. In this paper we are analytically analyzing the formation of electrostatic complexes between negatively charged ssDNA and positively charged iron oxide nanoparticles (SPIO-NP) with the purpose of identifying the optimal conditions leading to stable formulations at high oligo loading levels. The formation and loading levels of ssDNA:SPIO-NP complexes have been investigated at different oligo:NP ratios and under different ionic strengths through dynamic light scattering, fluorescence quenching experiments, and pull-down assays. Through these studies we have identified optimal conditions for attaining maximal oligo loading levels, and we are proposing a simple model to explain an unusual behavior observed in the formation of the complexes. Finally, we introduce an alternative loading method relying on the electrostatic coloading of an Ago sequence in the presence of a negatively charged PEGylated block copolymer, yielding very stable and high loading PEGylated ssDNA:SPIO-NPs. The findings that we are reporting are of general validity, and similar conditions could be easily translated to the electrostatic formation of ssDNA:NP complexes consisting of different NP materials and sizes.
引用
收藏
页码:18293 / 18299
页数:7
相关论文
共 50 条
  • [31] Fatty acid coated iron oxide nanoparticle: Effect on stability, particle size and magnetic properties
    Ong, Hun Tiar
    Suppiah, Durga Devi
    Julkapli, Nurhidayatullaili Muhd
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2020, 606
  • [32] The impact of ammonium hydroxide flow rate on iron oxide nanoparticle hydrodynamic size and colloidal stability
    Paige, Stephen
    Sedighi, Omid
    Stender, Ian
    Doiron, Amber L.
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2024, 702
  • [33] STUDY OF SURFACE COMPLEXES FORMED DURING PROPYLENE INTERACTION WITH CR-MO OXIDE CATALYST
    KUZNETSOV, VA
    BELOKOPYTOV, JV
    KONOVALOVA, ND
    GOROKHOVATSKIJ, JV
    DOPOVIDI AKADEMII NAUK UKRAINSKOI RSR SERIYA B-GEOLOGICHNI KHIMICHNI TA BIOLOGICHNI NAUKI, 1977, (11): : 991 - 994
  • [34] APICAL INTERACTION IN COPPER (II) COMPLEXES-STABILITY AND STRUCTURE OF THE TERNARY COPPER (II) COMPLEXES FORMED WITH DIETHYLENETRIAMINE AND PEPTIDES
    SHOUKRY, MM
    DARWISH, NA
    HOSNY, WM
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1986, 192 : 335 - INOR
  • [35] Iron oxide nanoparticle biosynthesis and Potential application for hepatic cellular damages through antioxidant stress
    Gul, Hina
    Irum, Samra
    Ahmed, Warda
    Awais, Muhammad
    Khan, M. Ijaz
    BIOMASS CONVERSION AND BIOREFINERY, 2023,
  • [36] Optimising titanium implant stability and infection resistance through iron nanoparticle coatings: A preclinical investigation
    Nelogi, Santosh
    Kumarpatil, Anand
    Chowdhary, Ramesh
    Roy, Richa
    JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY, 2025, 126 (06)
  • [37] Triggered Release from Liposomes through Magnetic Actuation of Iron Oxide Nanoparticle Containing Membranes
    Amstad, Esther
    Kohlbrecher, Joachim
    Mueller, Elisabeth
    Schweizer, Thomas
    Textor, Marcus
    Reimhult, Erik
    NANO LETTERS, 2011, 11 (04) : 1664 - 1670
  • [38] Multimodal biomedical imaging with asymmetric single-walled carbon nanotube/iron oxide nanoparticle complexes
    Choi, Jong Hyun
    Nguyen, Freddy T.
    Barone, Paul W.
    Heller, Daniel A.
    Moll, Anthonie E.
    Patel, Dhaval
    Boppart, Stephen A.
    Strano, Michael S.
    NANO LETTERS, 2007, 7 (04) : 861 - 867
  • [39] Dendrimer modified magnetic iron oxide nanoparticle/dna/pei ternary complexes: A novel strategy for magnetofection
    Liu, Wen-Ming
    Xue, Ya-Nan
    He, Wen-Tao
    Zhuo, Ren-Xi
    Huang, Shi-Wen
    JOURNAL OF CONTROLLED RELEASE, 2011, 152 : E159 - E160
  • [40] Effect of Grafted Copolymer Composition on Iron Oxide Nanoparticle Stability and Transport in Porous Media at High Salinity
    Xue, Zheng
    Foster, Edward
    Wang, Yonggang
    Nayak, Susheela
    Cheng, Victoria
    Ngost, Vincent W.
    Pennell, Kurt D.
    Bielawski, Christopher W.
    Johnston, Keith P.
    ENERGY & FUELS, 2014, 28 (06) : 3655 - 3665