A group of four series (A-D) of 22 new bioactive 1-N-acid substituted 3, 5-diphenyl-2-pyrazolines were synthesized by cyclization of variably substituted chalcones and simple or substituted phenyl hydrazine and / or semicarbazide, using acetic acid as a solvent. The chemical structure of the compounds was characterized by FTIR, (HNMR)-H-1, and EIMS spectroscopy and chemical analyses. The antifungal and antibacterial activities of these compounds were evaluated by agar tube dilution method and agar well diffusion method respectively.