Comparison of Plasmid DNA preparation methods for direct gene transfer and genetic immunization

被引：0
|
作者：
Davis, HL
Schleef, M
Moritz, P
Mancini, M
Schorr, J
Whalen, RG
机构：
[1] UNIV OTTAWA,OTTAWA,ON K1N 6N5,CANADA
[2] QIAGEN GMBH,HILDEN,GERMANY
[3] INST PASTEUR,PARIS,FRANCE
[4] COLL FRANCE,F-75231 PARIS,FRANCE
来源：
BIOTECHNIQUES | 1996年 / 21卷 / 01期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.
引用
收藏
页码：92 / &
页数：6
相关论文
共 50 条
  • [21] Genetic immunization of schistosomiasis using DNA plasmid encoding for key glycolytic enzyme
    Saber, MA
    El Dabaa, E
    FEBS JOURNAL, 2005, 272 : 566 - 567
  • [22] Genetic immunization and antibody generation in research animals by intravenous delivery of plasmid DNA
    Bates, MK
    Zhang, GF
    Sebestyen, MG
    Neal, ZC
    Wolff, JA
    Herweijer, H
    JOURNAL OF IMMUNOTHERAPY, 2005, 28 (06) : 643 - 643
  • [23] Genetic immunization for antibody generation in research animals by intravenous delivery of plasmid DNA
    Bates, MK
    Zhang, GF
    Sebestyén, MG
    Neal, ZC
    Wolff, JA
    Herweijer, H
    BIOTECHNIQUES, 2006, 40 (02) : 199 - 208
  • [24] Direct colony sequencing of plasmid DNA by dye terminator methods
    Sasho, C
    BIOTECHNIQUES, 2000, 29 (03) : 442 - +
  • [25] A comparison of methods for direct gene transfer into maize (Zea mays L.)
    Southgate, EM
    Davey, MR
    Power, JB
    Westcott, RJ
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 1998, 34 (03) : 218 - 224
  • [26] A comparison of methods for direct gene transfer into maize (Zea mays L.)
    Southgate E.M.
    Davey M.R.
    Power J.B.
    Westcott R.J.
    In Vitro Cellular & Developmental Biology - Plant, 1998, 34 (3) : 218 - 224
  • [27] Vertical and horizontal gene transfer tradeoffs direct plasmid fitness
    Bethke, Jonathan H.
    Ma, Helena R.
    Tsoi, Ryan
    Cheng, Li
    Xiao, Minfeng
    You, Lingchong
    MOLECULAR SYSTEMS BIOLOGY, 2023, 19 (02)
  • [28] Downstream processing of plasmid DNA for gene therapy and genetic vaccination
    Voss, Carsten
    CHEMICAL ENGINEERING & TECHNOLOGY, 2008, 31 (06) : 858 - 863
  • [29] Genetic immunization with plasmid DNA coding for the ROP2 protein of Toxoplasma gondii
    Leyva, R
    Hérion, P
    Saavedra, R
    PARASITOLOGY RESEARCH, 2001, 87 (01) : 70 - 79
  • [30] Therapeutic Option of Plasmid-DNA Based Gene Transfer
    Taniyama, Yoshiaki
    Azuma, Junya
    Kunugiza, Yasuo
    Iekushi, Kazuma
    Rakugi, Hiromi
    Morishita, Ryuichi
    CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2012, 12 (15) : 1630 - 1637
12345