Comparison of Plasmid DNA preparation methods for direct gene transfer and genetic immunization

被引：0
|
作者：
Davis, HL
Schleef, M
Moritz, P
Mancini, M
Schorr, J
Whalen, RG
机构：
[1] UNIV OTTAWA,OTTAWA,ON K1N 6N5,CANADA
[2] QIAGEN GMBH,HILDEN,GERMANY
[3] INST PASTEUR,PARIS,FRANCE
[4] COLL FRANCE,F-75231 PARIS,FRANCE
来源：
BIOTECHNIQUES | 1996年 / 21卷 / 01期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.
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页码：92 / &
页数：6
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