Differentiation of Candida glabrata, C. nivariensis and C. bracarensis based on fragment length polymorphism of ITS1 and ITS2 and restriction fragment length polymorphism of ITS and D1/D2 regions in rDNA

被引：20

作者：

Mirhendi, H. ^{[1
,2
,3
]}

Bruun, B. ^{[4
]}

Schonheyder, H. C. ^{[5
]}

Christensen, J. J. ^{[6
]}

Fuursted, K. ^{[7
]}

Gahrn-Hansen, B. ^{[8
]}

Johansen, H. K. ^{[9
]}

Nielsen, L. ^{[10
]}

Knudsen, J. D. ^{[11
]}

Arendrup, M. C. ^{[1
]}

机构：

[1] State Serum Inst, Unit Mycol & Parasitol, Copenhagen, Denmark

[2] Univ Tehran Med Sci, Dept Med Mycol & Parasitol, Sch Publ Hlth, Tehran, Iran

[3] Univ Tehran Med Sci, Natl Inst Hlth Res, Tehran, Iran

[4] Cent Hosp Hillerod, Dept Clin Microbiol, Hillerod, Denmark

[5] Aarhus Univ Hosp, Aalborg Hosp, Dept Clin Microbiol, Aalborg, Denmark

[6] Statens Serum Inst, Unit Clin Microbiol, DK-2300 Copenhagen, Denmark

[7] Aarhus Univ Hosp, Skejby Hosp, Dept Clin Microbiol, DK-8000 Aarhus, Denmark

[8] Odense Univ Hosp, Dept Clin Microbiol, DK-5000 Odense, Denmark

[9] Copenhagen Univ Hosp, Rigshosp, Dept Clin Microbiol, Copenhagen, Denmark

[10] Herlev Univ Hosp, Dept Clin Microbiol, DK-2730 Herlev, Denmark

[11] Hvidovre Univ Hosp, Dept Clin Microbiol, DK-2650 Hvidovre, Denmark

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2011年 / 30卷 / 11期

关键词：

RAPID IDENTIFICATION; SEQUENCE-ANALYSIS; SP-NOV; YEAST; PCR; SUSCEPTIBILITY; ORTHOPSILOSIS; METAPSILOSIS; FUNGEMIA; FUNGUS;

D O I：

10.1007/s10096-011-1235-9

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Different molecular methods for the discrimination of Candida glabrata, C. bracarensis and C. nivariensis were evaluated and the prevalence of these species among Danish blood isolates investigated. Control strains were used to determine fragment length polymorphism in the ITS1, ITS2, ITS1-5.8S-ITS2 regions and in the D1/D2 domain of 26S rDNA using primers designed for this study. A total of 133 blood isolates previously identified as C. glabrata were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH) method. The size of ITS1 allowed differentiation between C. glabrata (483), C. nivariensis (361) and C. bracarensis (385), whereas the ITS2 region was of similar size in C. nivariensis (417) and C. glabrata (418). Sequence analysis of the ITS region suggested that many restriction enzymes were suitable for RFLP differentiation of the species. Enzymatic digestion of the D1/D2 domain with TatI produced unique band sizes for each of the three species. PCR-RFLP and PNA-FISH were in agreement for all of the isolates tested. None of the 133 Danish blood isolates were C. nivariensis or C. bracarensis. Fragment size polymorphism of ITS1 and RFLP of the D1/D2 domain or the ITS region are useful methods for the differentiation of the species within the C. glabrata group. C. bracarensis and C. nivariensis are rare among Danish C. glabrata blood isolates.

引用

页码：1409 / 1416

页数：8

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