Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds

被引：5
|
作者：
Alsharabasy, Amir M. [1 ]
Pandit, Abhay [1 ]
机构：
[1] Natl Univ Ireland Galway, SFI Res Ctr Med Devices, CURAM, Galway H91 W2TY, Ireland
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 01期
基金：
爱尔兰科学基金会;
关键词：
Dermal fibroblast; Alsharabasy & amp; Pandit; STAR; LUNG FIBROSIS; ACTIN;
D O I：
10.1016/j.xpro.2021.100387
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Controversies remain over the standard procedures for the modeling of skin fibrosis and its use in in vitro testing of different drugs. Here, we report a repro-ducible protocol for producing a skin fibrosis model using human dermal fibro-blasts seeded in collagen hydrogel. Detailed procedures for the fabrication of cell/hydrogel constructs, fibrosis induction, protein extraction for western blot-ting analysis are presented along with how this model can be employed for inves-tigating the possible anti-fibrotic functions of certain chemical compounds.
引用
收藏
页数：13
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