Protocol for in vitro skin fibrosis model to screen the biological effects of antifibrotic compounds

被引：5

作者：

Alsharabasy, Amir M. ^{[1
]}

Pandit, Abhay ^{[1
]}

机构：

[1] Natl Univ Ireland Galway, SFI Res Ctr Med Devices, CURAM, Galway H91 W2TY, Ireland

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 01期

基金：

爱尔兰科学基金会;

关键词：

Dermal fibroblast; Alsharabasy & amp; Pandit; STAR; LUNG FIBROSIS; ACTIN;

D O I：

10.1016/j.xpro.2021.100387

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Controversies remain over the standard procedures for the modeling of skin fibrosis and its use in in vitro testing of different drugs. Here, we report a repro-ducible protocol for producing a skin fibrosis model using human dermal fibro-blasts seeded in collagen hydrogel. Detailed procedures for the fabrication of cell/hydrogel constructs, fibrosis induction, protein extraction for western blot-ting analysis are presented along with how this model can be employed for inves-tigating the possible anti-fibrotic functions of certain chemical compounds.

引用

页数：13

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