Characterization of the antigenicity of Cpl1, a surface protein of Cryptococcus neoformans var. neoformans

被引：6
|
作者：
Cai, Jian-Piao [1 ]
Liu, Ling-Li [2 ,3 ]
To, Kelvin Kw. [4 ]
Lau, Candy C. Y. [5 ]
Woo, Patrick C. Y. [4 ]
Lau, Susanna K. P. [4 ]
Guo, Yong-Hui [2 ]
Ngan, Antonio H. Y. [5 ]
Che, Xiao-Yan [2 ]
Yuen, Kwok-Yung [4 ]
机构：
[1] Univ Hong Kong, Dept Microbiol, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[2] Southern Med Univ, Zhujiang Hosp, Ctr Clin Lab, Guangzhou, Guangdong, Peoples R China
[3] Hainan Prov Peoples Hosp, Dept Clin Lab, Haikou, Peoples R China
[4] Univ Hong Kong, Res Ctr Infect & Immunol, Carol Yu Ctr Infect, Dept Microbiol,State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[5] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
来源：
MYCOLOGIA | 2015年 / 107卷 / 01期
关键词：
capsule; CLP1; Cryptococcus; fungus; immunostaining; CAPSULAR POLYSACCHARIDE; MONOCLONAL-ANTIBODIES; CEREBROSPINAL-FLUID; FUNGAL PATHOGEN; INFECTION; IMMUNOASSAY; MENINGITIS; PATIENT;
D O I：
10.3852/14-074
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Cryptococcus neoformans var. neoformans is an important fungal pathogen. The capsule is a well established virulence factor and a target site for diagnostic tests. The CPL1 gene is required for capsular formation and virulence. The protein product Cpl1 has been proposed to be a secreted protein, but the characteristics of this protein have not been reported. Here we sought to characterize Cpl1. Phylogenetic analysis showed that the Cpl1 of C. neoformans var. neoformans and the Cpl1 orthologs identified in C. neoformans var. grubii and C. gattii formed a distinct cluster among related fungi; while the putative ortholog found in Trichosporon asahii was distantly related to the Cryptococcus cluster. We expressed Cpl1 abundantly as a secreted His-tagged protein in Pichia pastoris. The protein was used to immunize guinea pigs and rabbits for high titer mono-specific polyclonal antibody that was shown to be highly specific against the cell wall of C. neoformans var. neoformans and did not cross react with C. gattii, T. asahii, Aspergillus spp., Candida spp. and Penicillium spp. Using the anti-Cpl1 antibody, we detected Cpl1 protein in the fresh culture supernatant of C. neoformans var. neoformans and we showed by immunostaining that the Cpl1 protein was located on the surface. The Cpl1 protein is a specific surface protein of C. neoformans var. neoformans.
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收藏
页码：39 / 45
页数：7
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