Identification of the internal axial ligand of HO2-cobalt(III)-bleomycin:: 1H{15N} HSQC NMR investigation of bleomycin, deglycobleomycin, and their hydroperoxide-cobalt(III) complexes

被引：15

作者：

Xia, CW ^{[1
]}

Försterling, FH ^{[1
]}

Petering, DH ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Chem, Milwaukee, WI 53201 USA

来源：

BIOCHEMISTRY | 2003年 / 42卷 / 21期

关键词：

D O I：

10.1021/bi030016c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The identity of the axial ligand contributed by the drug in hydroperoxide-Co(III)-bleomycin and hydroperoxide-Co(III)-deglycobleomycin has been in doubt. With each structure, a combination of H-1{N-15} HSQC and HMBC and H-1 COSY and NOESY NMR spectroscopy was used to observe and completely assign the nonaromatic N-15 chemical shifts of natural abundance bleomycin in the two hydroperoxide-Co(III) structures. Together with the N-15 assignments from a published ID N-15 spectrum, the results permitted the assignment of the primary amine nitrogen to an axial ligand position in both structures.

引用

页码：6559 / 6564

页数：6

共 22 条

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