Simultaneous determination of lidocaine, proline and lomefloxacin in human urine by CE with electrochemiluminescence detection

A new method was developed for the simultaneous determination of lidocaine, proline and lomefloxacin in human urine by capillary electrophoresis-electrochemiluminescence detection with Ru(bpy)(3)(2+). Conditions of the separation and detection were investigated and optimized. It was proved that 20 mM phosphate buffer at pH 6.7 could achieve the most favorable resolution, and the high sensitivity of detection was obtained by using the detection potential at 1.15 V and 5 mM Ru(bpy)(3)(2+)-60 mM phosphate buffer at pH 7.6 in the detection reservoir. The detection limits were 0.02 mu g mL(-1) for lidocaine, 0.03 mu g mL(-1) for proline and 0.06 mu g mL(-1) for lomefloxacin. Relative standard deviations of the ECL intensity and the migration time were 3.5 and 1.1% for 6 mu g mL(-1) lidocaine, 3.2 and 1.0% for 6 mu g mL(-1) proline and 3.7 and 1.2% for 6 mu g mL(-1) lomefloxacin, respectively. A baseline separation for lidocaine, proline and lomefloxacin was achieved within 360 s. The developed method was successfully applied to determine the amounts of lidocaine, proline and lomefloxacin in human urine. The recovery and RSD were in the range of 93.3-97.2 and 3.8-4.9%, respectively.