The role of L-type amino acid transporter 1 (Slc7a5) during in vitro myogenesis

被引:9
|
作者
Collao, Nicolas [1 ]
Akohene-Mensah, Paul [1 ]
Nallabelli, Julian [2 ]
Binet, Emileigh R. [1 ]
Askarian, Ali [1 ]
Lloyd, Jessica
Niemiro, Grace M. [2 ]
Beals, Joseph W. [2 ]
van Vliet, Stephan [2 ]
Rajgara, Rashida [3 ]
Saleh, Aisha [3 ]
Wiper-Bergeron, Nadine [3 ]
Paluska, Scott A. [2 ]
Burd, Nicholas A. [2 ]
De Lisio, Michael [1 ,2 ,3 ]
机构
[1] Univ Ottawa, Sch Human Kinet, Ottawa, ON, Canada
[2] Univ Illinois, Dept Kinesiol & Community Hlth, Urbana, IL 61820 USA
[3] Univ Ottawa, Ctr Neuromuscular Dis, Dept Cellular & Mol Med, Ottawa, ON, Canada
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2022年 / 323卷 / 02期
基金
加拿大自然科学与工程研究理事会;
关键词
amino acids; leucine; myoblast; protein; satellite cells; MUSCLE PROTEIN-SYNTHESIS; SKELETAL-MUSCLE; SATELLITE CELLS; STEM-CELLS; EXPRESSION; LEUCINE; DIFFERENTIATION; PROLIFERATION; STIMULATION; MOUSE;
D O I
10.1152/ajpcell.00162.2021
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Satellite cells are required for muscle regeneration, remodeling, and repair through their activation, proliferation, and differentiation; however, how dietary factors regulate this process remains poorly understood. The L-type amino acid transporter 1 (LAT1) transports amino acids, such as leucine, into mature myofibers, which then stimulate protein synthesis and anabolic signaling. However, whether LAT1 is expressed on myoblasts and is involved in regulating myogenesis is unknown. The aim of this study was to characterize the expressional and functional relevance of LAT1 during different stages of myogenesis and in response to growth and atrophic conditions in vitro. We determined that LAT1 is expressed by C2C12 and human primary myoblasts, and its gene expression is lower during differentiation (P < 0.05). Pharmacological inhibition and genetic knockdown of LAT1 impaired myoblast viability, differentiation, and fusion (all P < 0.05). LAT1 protein content in C2C12 myoblasts was not significantly altered in response to different leucine concentrations in cell culture media or in two in vitro atrophy models. However, LAT1 content was decreased in myotubes under atrophic conditions in vitro (P < 0.05). These findings indicate that LAT1 is stable throughout myogenesis and in response to several in vitro conditions that induce muscle remodeling. Further, amino acid transport through LAT1 is required for normal myogenesis in vitro.
引用
收藏
页码:C595 / C605
页数:11
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