Light sheet fluorescence microscopy using high-speed structured and pivoting illumination

被引：13
|
作者：
Itoh, Ryosuke [1 ]
Landry, Joseph Russell [2 ]
Hamann, Stephen Sanborn [2 ]
Solgaard, Olav [2 ]
机构：
[1] SCREEN Holdings Co Ltd, R&D Dept, Fushimi Ku, 322 Furukawa Cho, Kyoto 6128486, Japan
[2] Stanford Univ, Edward L Ginzton Lab, Stanford, CA 94305 USA
来源：
OPTICS LETTERS | 2016年 / 41卷 / 21期
关键词：
D O I：
10.1364/OL.41.005015
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We show that light sheet fluorescence microscopy with structured and pivoting illumination enables fast image acquisition and improved image quality. A one-dimensional spatial light phase modulator is used to control the illumination profile at high speed. To demonstrate the features of the system, we image fluorescent beads and biological samples, successfully obtaining optically sectioned images with higher contrast using structured illumination and with reduced shadowing effects using pivoting illumination. (C) 2016 Optical Society of America
引用
下载
收藏
页码：5015 / 5018
页数：4
相关论文
共 50 条
  • [21] High-speed image reconstruction for optically sectioned, super-resolution structured illumination microscopy
    Wang, Zhaojun
    Zhao, Tianyu
    Hao, Huiwen
    Cai, Yanan
    Feng, Kun
    Yun, Xue
    Liang, Yansheng
    Wang, Shaowei
    Sun, Yujie
    Bianco, Piero R.
    Oh, Kwangsung
    Lei, Ming
    ADVANCED PHOTONICS, 2022, 4 (02):
  • [22] Speed limits of structured illumination microscopy
    Strohl, Florian
    Kaminski, Clemens F.
    OPTICS LETTERS, 2017, 42 (13) : 2511 - 2514
  • [23] High speed structured illumination microscopy in optically thick samples
    Shaw, Michael
    Zajiczek, Lydia
    O'Holleran, Kevin
    METHODS, 2015, 88 : 11 - 19
  • [24] Application of Structured Light Illumination and Compressed Sensing to High Speed Laminar Optical Fluorescence Tomography
    Sakuma, Ichiro
    Kobayashi, Toshiki
    Seno, Hiroshi
    Akagi, Yuki
    Nakagawa, Keiichi
    Yamazaki, Masatoshi
    Tomii, Naoki
    VIII LATIN AMERICAN CONFERENCE ON BIOMEDICAL ENGINEERING AND XLII NATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING, 2020, 75 : 1216 - 1219
  • [25] Stripe artifact reduction for digital scanned structured illumination light sheet microscopy
    Liu, Yang
    Lauderdale, James D.
    Kner, Peter
    OPTICS LETTERS, 2019, 44 (10) : 2510 - 2513
  • [26] High-resolution fluorescence microscopy of retinal pigment epithelium using structured illumination
    Ach, T.
    Best, G.
    Ruppenstein, M.
    Amberger, R.
    Cremer, C.
    Dithmar, S.
    OPHTHALMOLOGE, 2010, 107 (11): : 1037 - 1042
  • [27] High-Speed Compressive Microscopy of Flowing Cells Using Sinusoidal Illumination Patterns
    Guo, Qiang
    Chen, Hongwei
    Wang, Yuxi
    Guo, Yong
    Liu, Peng
    Zhu, Xiurui
    Cheng, Zhen
    Yu, Zhenming
    Yang, Sigang
    Chen, Minghua
    Xie, Shizhong
    IEEE PHOTONICS JOURNAL, 2017, 9 (01):
  • [28] High-speed fluorescence microscopy of cell signaling
    Petty, HR
    26TH INTERNATIONAL CONGRESS ON HIGH SPEED PHOTOGRAPHY AND PHOTONICS, 2005, 5580 : 81 - 92
  • [29] csiLSFM combines light- sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm
    Chang, Bo-Jui
    Meza, Victor Didier Perez
    Stelzer, Ernst H. K.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (19) : 4869 - 4874
  • [30] High-speed super-resolution imaging with compressive imaging-based structured illumination microscopy
    He, Yilin
    Yao, Yunhua
    Qi, Dalong
    Wang, Zhiyong
    Jia, Tianqing
    Liang, Jinyang
    Sun, Zhenrong
    Zhang, Shian
    OPTICS EXPRESS, 2022, 30 (09): : 14287 - 14299
12345