Light sheet fluorescence microscopy using high-speed structured and pivoting illumination

被引：13
|
作者：
Itoh, Ryosuke [1 ]
Landry, Joseph Russell [2 ]
Hamann, Stephen Sanborn [2 ]
Solgaard, Olav [2 ]
机构：
[1] SCREEN Holdings Co Ltd, R&D Dept, Fushimi Ku, 322 Furukawa Cho, Kyoto 6128486, Japan
[2] Stanford Univ, Edward L Ginzton Lab, Stanford, CA 94305 USA
来源：
OPTICS LETTERS | 2016年 / 41卷 / 21期
关键词：
D O I：
10.1364/OL.41.005015
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We show that light sheet fluorescence microscopy with structured and pivoting illumination enables fast image acquisition and improved image quality. A one-dimensional spatial light phase modulator is used to control the illumination profile at high speed. To demonstrate the features of the system, we image fluorescent beads and biological samples, successfully obtaining optically sectioned images with higher contrast using structured illumination and with reduced shadowing effects using pivoting illumination. (C) 2016 Optical Society of America
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页码：5015 / 5018
页数：4
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